Bussmann L E, Koninckx A, Deis R P
Biol Reprod. 1983 Oct;29(3):535-41. doi: 10.1095/biolreprod29.3.535.
The removal of the corpora lutea or ovariectomy on Day 18 of pregnancy induced a rise in serum prolactin 24 h after surgery with a rapid decline to control values 4 h after the surge, only in the ovariectomized group. When hysterectomy was performed in addition to luteectomy or ovariectomy a similar rise in prolactin was obtained. Lactose synthetase activity in mammary tissue was significantly higher in the luteectomized and luteectohysterectomized rats when compared with ovariectomized, ovariohysterectomized rats and the sham-operated group. Estrogen treatment 12 h after ovariectomy increased serum prolactin and lactose synthetase activity to values similar to those measured in luteectomized rats, but this increase was significantly greater when compared with the ovariectomized-nontreated group. Treatment with Tamoxifen did not decrease serum prolactin in the luteectomized rats but lactose synthetase was reduced to values similar to that obtained in ovariectomized rats. Treatment with 2 bromo-alpha-ergocryptine-mesylate (CB-154) prevented the rise in serum prolactin in the ovariectomized, luteectomized and luteectohysterectomized groups, but lactose synthetase activity was lowered to control values (sham-operated rats) only in the luteectohysterectomized rats. According to these findings, rat placental lactogen in the absence of prolactin and progesterone induces lactose synthesis. Estrogen facilitates prolactin but not placental lactogen action on lactose synthetase activity.
在妊娠第18天切除黄体或进行卵巢切除术，仅在卵巢切除组中，术后24小时血清催乳素水平升高，在激增后4小时迅速降至对照值。当除了切除黄体或卵巢之外还进行子宫切除术时，催乳素也会出现类似的升高。与卵巢切除、卵巢子宫切除大鼠及假手术组相比，切除黄体和切除黄体子宫切除的大鼠乳腺组织中的乳糖合成酶活性显著更高。卵巢切除术后12小时给予雌激素治疗，可使血清催乳素和乳糖合成酶活性升高至与切除黄体大鼠中测得的值相似，但与未治疗的卵巢切除组相比，这种升高显著更大。用他莫昔芬治疗并未降低切除黄体大鼠的血清催乳素，但乳糖合成酶降至与卵巢切除大鼠中获得的值相似。用2-溴-α-麦角隐亭甲磺酸盐（CB-154）治疗可防止卵巢切除、切除黄体和切除黄体子宫切除组中血清催乳素的升高，但仅在切除黄体子宫切除的大鼠中乳糖合成酶活性降至对照值（假手术大鼠）。根据这些发现，在没有催乳素和孕酮的情况下，大鼠胎盘催乳素可诱导乳糖合成。雌激素促进催乳素对乳糖合成酶活性的作用，但不促进胎盘催乳素的作用。
