遗传感受态细菌膜中的 D-(-)-聚-β-羟基丁酸酯
D-(-)-poly-beta-hydroxybutyrate in membranes of genetically competent bacteria.
作者信息
Reusch R N, Sadoff H L
出版信息
J Bacteriol. 1983 Nov;156(2):778-88. doi: 10.1128/jb.156.2.778-788.1983.
Abstract
D-(-)-Poly-beta-hydroxybutyrate is a constituent of the membranes and the cytoplasms of genetically competent Azotobacter vinelandii, Bacillus subtilis, and Haemophilus influenzae cells. Within each species the concentration of D-(-)-poly-beta-hydroxybutyrate in the membranes and cytoplasm correlates with transformability. Fluorescence analysis of the thermotropic lipid phase transitions in A. vinelandii and B. subtilis cells indicates that D-(-)-poly-beta-hydroxybutyrate forms an organized gel structure in the membranes which is very labile. The concentration of organized D-(-)-poly-beta-hydroxybutyrate in the membranes, which can be estimated from the intensity of its phase transition, can be used to assess the competence of a culture.
摘要
D-(-)-聚-β-羟基丁酸酯是具有遗传转化能力的棕色固氮菌、枯草芽孢杆菌和流感嗜血杆菌细胞膜及细胞质的组成成分。在每个物种中，细胞膜和细胞质中D-(-)-聚-β-羟基丁酸酯的浓度与转化能力相关。对棕色固氮菌和枯草芽孢杆菌细胞热致脂质相变的荧光分析表明，D-(-)-聚-β-羟基丁酸酯在细胞膜中形成一种非常不稳定的有序凝胶结构。可根据其相变强度估算的细胞膜中有序D-(-)-聚-β-羟基丁酸酯的浓度，可用于评估培养物的转化能力。
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本文引用的文献
1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Isolation and identification of the sudanophilic granules of Sphaerotilus natans.浮游球衣菌嗜苏丹颗粒的分离与鉴定。
J Bacteriol. 1962 Feb;83(2):343-7. doi: 10.1128/jb.83.2.343-347.1962.
4
Assay of poly-beta-hydroxybutyric acid.聚-β-羟基丁酸的测定
J Bacteriol. 1961 Jul;82(1):33-6. doi: 10.1128/jb.82.1.33-36.1961.
5
The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.大肠杆菌B提取物中2-酮-3-脱氧庚糖酸的形成。I. 鉴定
J Biol Chem. 1959 Apr;234(4):705-9.
6
The localisation of small molecules in lipid bilayers.小分子在脂质双层中的定位。
FEBS Lett. 1972 Aug 15;24(3):241-246. doi: 10.1016/0014-5793(72)80364-8.
7
Genetic transformation.遗传转化
Annu Rev Biochem. 1981;50:41-68. doi: 10.1146/annurev.bi.50.070181.000353.
8
Transformation-deficient mutants of Bacillus subtilis impaired in competence-specific nuclease activities.枯草芽孢杆菌的转化缺陷型突变体在感受态特异性核酸酶活性方面存在缺陷。
J Bacteriol. 1982 Oct;152(1):166-74. doi: 10.1128/jb.152.1.166-174.1982.
9
Modified spray for the detection of phospholipids on thin-layer chromatograms.用于检测薄层色谱图上磷脂的改良喷雾剂。
J Lipid Res. 1968 May;9(3):396.
10
Defined nongrowth media for stage II development of competence in Haemophilus influenzae.用于流感嗜血杆菌感受态II期发育的限定非生长培养基。
J Bacteriol. 1970 Feb;101(2):517-24. doi: 10.1128/jb.101.2.517-524.1970.
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