Reusch R N, Sadoff H L
J Bacteriol. 1983 Nov;156(2):778-88. doi: 10.1128/jb.156.2.778-788.1983.
D-(-)-Poly-beta-hydroxybutyrate is a constituent of the membranes and the cytoplasms of genetically competent Azotobacter vinelandii, Bacillus subtilis, and Haemophilus influenzae cells. Within each species the concentration of D-(-)-poly-beta-hydroxybutyrate in the membranes and cytoplasm correlates with transformability. Fluorescence analysis of the thermotropic lipid phase transitions in A. vinelandii and B. subtilis cells indicates that D-(-)-poly-beta-hydroxybutyrate forms an organized gel structure in the membranes which is very labile. The concentration of organized D-(-)-poly-beta-hydroxybutyrate in the membranes, which can be estimated from the intensity of its phase transition, can be used to assess the competence of a culture.
D-(-)-聚-β-羟基丁酸酯是具有遗传转化能力的棕色固氮菌、枯草芽孢杆菌和流感嗜血杆菌细胞膜及细胞质的组成成分。在每个物种中，细胞膜和细胞质中D-(-)-聚-β-羟基丁酸酯的浓度与转化能力相关。对棕色固氮菌和枯草芽孢杆菌细胞热致脂质相变的荧光分析表明，D-(-)-聚-β-羟基丁酸酯在细胞膜中形成一种非常不稳定的有序凝胶结构。可根据其相变强度估算的细胞膜中有序D-(-)-聚-β-羟基丁酸酯的浓度，可用于评估培养物的转化能力。
