用噬菌体φdo7 DNA转染枯草芽孢杆菌原生质体。
Transfection of Bacillus subtilis protoplasts by bacteriophage phi do7 DNA.
作者信息
Perkins J B, Dean D H
出版信息
J Bacteriol. 1983 Nov;156(2):931-3. doi: 10.1128/jb.156.2.931-933.1983.
Abstract
DNA from the Bacillus subtilis temperate bacteriophage phi do7 was found to efficiently transfect B. subtilis protoplasts; protoplast transfection was more efficient than competent cell transfection by a magnitude of 10(3). Unlike competent cell transfection, protoplast transfection did not require primary recombination, suggesting that phi do7 DNA enters the protoplast as double-stranded molecules.
摘要
已发现来自枯草芽孢杆菌温和噬菌体phi do7的DNA能有效地转染枯草芽孢杆菌原生质体；原生质体转染比感受态细胞转染效率高10^3倍。与感受态细胞转染不同，原生质体转染不需要初次重组，这表明phi do7 DNA以双链分子形式进入原生质体。
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