White T B, Doyle R J, Streips U N
J Bacteriol. 1981 Feb;145(2):878-83. doi: 10.1128/jb.145.2.878-883.1981.
A stable L-form, sal-1, of Bacillus subtilis was transformed with deoxyribonucleic acid (DNA) from bacteriophages phi 25 and phi 29 to determine whether exogenous DNA can be introduced into this organism. The viral transformation (transfection) was successful with the use of polyethylene glycol. In the presence of the fusogen, bacteriophage phi 25 DNA initiated a single cycle of infection. When compared with transfection of competent cells of Bacillus subtilis, the appearance of viral particles was delayed and their production occurred over a longer time period. L-form cells were best able to support intracellular replication of phi 25 viral particles when in balanced growth in a rich medium. The addition of polyethylene glycol also induced infection of sal-1 with whole bacteriophage phi 25 particles which could not otherwise infect the L-form and enhanced infection by intact phi 29 particles. Primary recombination was shown to be required for polyethylene glycol-mediated phi 25 transfection, but not phi 29 transfection or for whole bacteriophage phi 25 infection mediated by polyethylene glycol. Successful transfection of sal-1 suggests that the L-form may be amenable to genetic modification with exogenous DNA.
用来自噬菌体φ25和φ29的脱氧核糖核酸（DNA）转化枯草芽孢杆菌的稳定L型菌株sal-1，以确定外源DNA是否能导入该生物体。利用聚乙二醇成功实现了病毒转化（转染）。在融合剂存在的情况下，噬菌体φ25 DNA引发了一个感染周期。与枯草芽孢杆菌感受态细胞的转染相比，病毒颗粒的出现延迟，且其产生持续更长时间。当在丰富培养基中处于平衡生长时，L型细胞最能支持φ25病毒颗粒的细胞内复制。聚乙二醇的添加还诱导了完整的噬菌体φ25颗粒对sal-1的感染，否则这些颗粒无法感染L型细胞，并增强了完整的φ29颗粒的感染。结果表明，聚乙二醇介导的φ25转染需要初级重组，但φ29转染或聚乙二醇介导的完整噬菌体φ25感染则不需要。sal-1的成功转染表明L型可能适合用外源DNA进行基因改造。
