Sutherland S S
Vet Res Commun. 1983 Sep;6(4):297-304. doi: 10.1007/BF02214925.
Forty-eight cattle were sensitised to Brucella antigens either by vaccination with Brucella abortus strain 19 (S19) or B. abortus 45/20 (S45/20) ad 24 of these and 12 unvaccinated cattle were subsequently challenged with virulent B. abortus strain 544 (S544). All these cattle (n = 60), together with 12 control cattle which were neither vaccinated nor challenged, were subsequently subjected to an intradermal test using a S45/20 protein antigen. Reactions were interpreted subjectively by observation and palpation and were measured to the nearest mm with calipers at 48 and 72 hours after injection of protein antigen. Ten weeks later the cattle were slaughtered and tissues cultured for the presence of B. abortus. Two of the 48 vaccinated cattle died, 40 of the remaining 46 gave a positive response to the intradermal test at 48 hours and 36 were positive at 72 hours. In the controls any increase in the skin thickness had disappeared by 72 hours. An increase in skin thickness was still present at 72 hours in all other cattle except those vaccinated with S19 only. The intradermal test was found to be sensitive but not specific in detecting infected cattle and both sensitive and highly specific if used (with the exception of S19) to detect exposure to Brucella antigen.
48头牛通过接种流产布鲁氏菌19号菌株（S19）或流产布鲁氏菌45/20菌株（S45/20）对布鲁氏菌抗原产生致敏，其中24头接种牛和12头未接种牛随后用强毒流产布鲁氏菌544菌株（S544）进行攻毒。所有这些牛（n = 60），连同12头既未接种也未攻毒的对照牛，随后使用S45/20蛋白抗原进行皮内试验。通过观察和触诊对反应进行主观判断，并在注射蛋白抗原后48小时和72小时用卡尺测量至最接近的毫米数。十周后，宰杀这些牛并对组织进行培养以检测是否存在流产布鲁氏菌。48头接种牛中有2头死亡，其余46头中的40头在48小时时对皮内试验呈阳性反应，36头在72小时时呈阳性反应。在对照牛中，皮肤厚度的任何增加在72小时时都已消失。除仅接种S19的牛外，所有其他牛在72小时时皮肤厚度仍有增加。发现皮内试验在检测感染牛时敏感但不特异，而如果用于（S19除外）检测接触布鲁氏菌抗原则既敏感又高度特异。
