Kawabata N, Sugiyama S, Kuwamura T, Odaka Y, Satoh T
J Pharm Sci. 1983 Oct;72(10):1162-5. doi: 10.1002/jps.2600721013.
A sensitive assay of both tegafur (I) and 5-fluorouracil (5-FU) using GLC with a nitrogen-phosphorus-sensitive detector is described. The drugs were extracted from rabbit serum with ethyl acetate and methylated with diazomethane. Linearity was obtained over the concentration ranges of 3.13-200 micrograms/ml for I and 0.0313-2 micrograms/ml and 10-50 ng/ml for 5-FU. The detection limits of I and 5-FU in serum were 50 and 8 ng/ml, respectively. The serum concentrations of the drugs determined by the present method closely agreed with those obtained by spectrophotometry for I and microbial assay for 5-FU.
描述了一种使用带有氮磷敏感检测器的气相色谱法（GLC）同时测定替加氟（I）和5-氟尿嘧啶（5-FU）的灵敏方法。药物用乙酸乙酯从兔血清中提取，并用重氮甲烷进行甲基化。I的浓度范围为3.13 - 200微克/毫升，5-FU的浓度范围为0.0313 - 2微克/毫升和10 - 50纳克/毫升时呈线性。血清中I和5-FU的检测限分别为50和8纳克/毫升。用本法测定的药物血清浓度与用分光光度法测定I和微生物法测定5-FU所得到的结果非常一致。
