在存在呋喃氟尿嘧啶的情况下用于测定血清氟尿嘧啶浓度的改进型液相色谱分析法。
Improved liquid chromatographic assay for serum fluorouracil concentrations in the presence of ftorafur.
作者信息
Hornbeck C L, Floyd R A, Griffiths J C, Byfield J E
出版信息
J Pharm Sci. 1981 Oct;70(10):1163-6. doi: 10.1002/jps.2600701021.
An improved liquid chromatographic assay for serum ftorafur and fluorouracil is shown to be routine, sensitive, and reproducible using 200 micro l of serum. Dilute ammonium acetate buffer at pH 10.2 is used for solubilization of the evaporated ethyl acetate extract for injection into the liquid chromatograph. A stability study indicated little or no in vitro formation of fluorouracil from ftorafur under the conditions described. Low serum fluorouracil levels were found after administration of therapeutic doses of ftorafur.
一种改进的血清喃氟啶和氟尿嘧啶液相色谱测定法显示，使用200微升血清时，该方法具有常规性、灵敏性和可重复性。pH 10.2的稀醋酸铵缓冲液用于溶解蒸发后的乙酸乙酯提取物，以便注入液相色谱仪。稳定性研究表明，在所描述的条件下，喃氟啶在体外很少或不会形成氟尿嘧啶。给予治疗剂量的喃氟啶后，血清氟尿嘧啶水平较低。
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