De Fabrizio F
J Pharm Sci. 1978 Apr;67(4):572-3. doi: 10.1002/jps.2600670440.
A simple chromatographic procedure was developed for the separation and determination of theophylline and hydroxyethyltheophylline. An aliquot of diluted syrup was pipetted into a chromatographic column containing a strong anion-exchange resin, which retains theophylline and allows the hydroxyethyl derivative to pass through it. Theophylline was subsequently eluted with 1 N HCl and determined by difference spectrophotometry at 284 nm; its derivative was determined at 272 nm.
开发了一种简单的色谱方法，用于分离和测定茶碱和羟乙基茶碱。将稀释糖浆的一份等分试样移液至装有强阴离子交换树脂的色谱柱中，该树脂保留茶碱并使羟乙基衍生物通过。随后用1 N HCl洗脱茶碱，并通过差示分光光度法在284 nm处测定；其衍生物在272 nm处测定。
