血浆中对乙酰氨基酚的定量测定。

Quantitative determination of acetaminophen in plasma.

作者信息

Plakogiannis F M, Saad A M

出版信息

J Pharm Sci. 1978 Apr;67(4):581. doi: 10.1002/jps.2600670444.

Abstract

A simple method is described for the rapid quantitative analysis of acetaminophen in plasma. The acetaminophen and its conjugates present in the plasma following drug administration are hydrolyzed with 4 N HCl to p-aminophenol. This compound is coupled with 5% vanillin reagent to form a stable yellow color whose concentration is determined spectrophotometrically at 395 nm. Application of this method to a study of three dogs treated with 650 mg of acetaminophen is described.

摘要

本文描述了一种用于血浆中对乙酰氨基酚快速定量分析的简单方法。给药后血浆中存在的对乙酰氨基酚及其结合物用4N盐酸水解为对氨基酚。该化合物与5%香草醛试剂偶联形成稳定的黄色，其浓度通过在395nm处进行分光光度测定。本文还描述了该方法在三只接受650mg对乙酰氨基酚治疗的狗的研究中的应用。