Manno B R, Manno J E, Dempsey C A, Wood M A
J Anal Toxicol. 1981 Jan-Feb;5(1):24-8. doi: 10.1093/jat/5.1.24.
N-Acetyl-p-aminophenol (acetaminophen) is becoming more prevalent as an intoxicant in accidental or intentional overdose, therefore, a direct injection ultra-micro high-pressure liquid chromatographic (HPLC) method has been developed for its quantitation. The HPLC analysis was performed using a Model 110 Solvent Metering Pump equipped with a Model 110-19 Pressure Filter (Altex Scientific, Berkeley, CA), a Model 7120 Rheodyne Injector (Rheodyne, Berkeley, CA) or a Model U6K Injector (Waters Associates, Milford, MA) a Model 440 Absorbance Detector (Water's Associates), and a Model 3380A Recorder Integrator (Hewlett Packard, Avondale, PA). A commercially prepared muBonapak C18 Column (Water's Associates) was used. Acetaminophen was eluted with a mixture of 0.01 mol/L aqueous sodium acetate, pH 4.0: acetonitrile (93:7) and the absorbance detector was operated wih a 254 nm filter. The method, which requires only 2 microL of serum or plasma for analysis, offers several distinct advantages to the analyst. No pre- or post-column extraction or other manipulation of the specimen is required to obtain a quantitative result. Rapid processing of the specimen is possible because both acetaminophen and the internal standard are eluted in less than 10 minutes. The small sample (2 microL) is ideal for use with pediatric patients.
N-乙酰对氨基酚（对乙酰氨基酚）作为意外或故意过量中毒的一种毒物正变得越来越普遍，因此，已开发出一种直接进样超微高压液相色谱（HPLC）法对其进行定量分析。HPLC分析使用配备110-19型压力过滤器（Altex Scientific公司，加利福尼亚州伯克利）的110型溶剂计量泵、7120型Rheodyne进样器（Rheodyne公司，加利福尼亚州伯克利）或U6K型进样器（沃特斯联合公司，马萨诸塞州米尔福德）、440型吸光度检测器（沃特斯联合公司）以及3380A型号记录积分仪（惠普公司，宾夕法尼亚州阿冯代尔）进行。使用市售的μBondapak C18柱（沃特斯联合公司）。对乙酰氨基酚用0.01mol/L乙酸钠水溶液（pH 4.0）与乙腈（93:7）的混合物洗脱，吸光度检测器使用254nm滤光片运行。该方法仅需2微升血清或血浆进行分析，为分析人员提供了几个明显的优势。获得定量结果无需对样品进行柱前或柱后萃取或其他处理。由于对乙酰氨基酚和内标物在不到10分钟内即可洗脱，因此可以快速处理样品。小样本量（2微升）非常适合儿科患者使用。
