Hammond P M, Scawen M D, Atkinson T, Campbell R S, Price C P
Anal Biochem. 1984 Nov 15;143(1):152-7. doi: 10.1016/0003-2697(84)90570-0.
A new and novel method for determination of serum acetaminophen is described. The assay, which can be completed in less than 5 min, is based on the enzymatic hydrolysis of acetaminophen, with subsequent colorimetric detection of the aminophenol so produced. Various possible means of aminophenol estimation are described; the final reaction conditions have been optimized for maximum sensitivity and assay speed. This assay compares favorably with other available procedures; it requires only small sample volumes; it is rapid, simple, and highly specific for the parent drug; and it requires neither great technical ability nor expensive instrumentation.
本文描述了一种测定血清对乙酰氨基酚的全新方法。该检测方法可在不到5分钟内完成，基于对乙酰氨基酚的酶促水解，随后对生成的氨基酚进行比色检测。文中描述了多种可能的氨基酚测定方法；最终的反应条件已针对最大灵敏度和检测速度进行了优化。该检测方法与其他现有方法相比具有优势；它仅需少量样本体积；快速、简单且对母体药物具有高度特异性；既不需要高超的技术能力，也不需要昂贵的仪器设备。
