Miles M A, Arias J R, Valente S A, Naiff R D, de Souza A A, Povoa M M, Lima J A, Cedillos R A
Am J Trop Med Hyg. 1983 Nov;32(6):1251-9. doi: 10.4269/ajtmh.1983.32.1251.
A total of 46 Trypanosoma rangeli stocks were isolated from naturally infected mammals and triatomine vectors. Twenty-two stocks were from the common opossum (Didelphis marsupialis), one from the brown "4-eyed" opossum (Metachirus nudicaudatus), one from the anteater (Tamandua tetradactyla), one from the coati (Nasua nasua), seven from Rhodnius pictipes and 14 from Rhodnius robustus. Two stocks were also isolated from recently fed sandflies (Lutzomyia sp., Shannoni group). The stocks were identified as T. rangeli on the basis of natural or experimental salivary gland infections in Rhodnius, inoculative (anterior station) transmission to mice, morphological parameters in parasitemic mice and comparisons of isozyme profiles with a known stock of T. rangeli isolated from man. Three other trypanosome stocks from D. marsupialis, T. tetradactyla and the three-toed sloth (Bradypus tridactylus) were morphologically similar to T. rangeli in culture but had quite different isozyme profiles and were not identified. It is concluded that T. rangeli is widely distributed in Amazonas, Pará and Rondonia States of Brazil, and probably extends into other regions where R. pictipes and R. robustus are known to occur. R. pictipes is light-attracted into houses and occasionally transmits Chagas' disease to man. It is likely that T. rangeli is also occasionally transmitted to man in the Amazon basin.
总共从自然感染的哺乳动物和锥蝽媒介中分离出46株兰氏锥虫。22株来自普通负鼠（Didelphis marsupialis），1株来自褐四眼负鼠（Metachirus nudicaudatus），1株来自食蚁兽（Tamandua tetradactyla），1株来自长鼻浣熊（Nasua nasua），7株来自饰边罗蛉（Rhodnius pictipes），14株来自粗壮罗蛉（Rhodnius robustus）。还从近期吸食过血液的白蛉（Lutzomyia sp.，香农尼组）中分离出2株。这些虫株根据罗蛉自然或实验性唾液腺感染、对小鼠的接种（前哨站）传播、患寄生虫血症小鼠的形态学参数以及与从人类分离出的已知兰氏锥虫虫株的同工酶谱比较，被鉴定为兰氏锥虫。另外3株分别来自普通负鼠、食蚁兽和三趾树懒（Bradypus tridactylus）的锥虫在培养中形态上与兰氏锥虫相似，但同工酶谱差异很大，未被鉴定。得出的结论是，兰氏锥虫广泛分布于巴西的亚马孙州、帕拉州和朗多尼亚州，可能还延伸至已知有饰边罗蛉和粗壮罗蛉存在的其他地区。饰边罗蛉被灯光吸引进入房屋，偶尔会将恰加斯病传播给人类。兰氏锥虫很可能也偶尔在亚马孙流域传播给人类。
