Póvoa M M, de Souza A A, Naiff R D, Arias J R, Naiff M F, Biancardi C B, Miles M A
Ann Trop Med Parasitol. 1984 Oct;78(5):479-87.
Of 151 Trypanosoma cruzi stocks from 117 different individual hosts collected in the states of Amazonas and Rondonia, 147 from 113 hosts were identified as zymodeme 1 (Z1). These included T. cruzi stocks from three marsupial species, two rodent species and three triatomine species although most were from the common opossum, Didelphis marsupialis. One T. cruzi stock from Rhodnius robustus was identified as Z1 with a Z3 PGM character, one from Sciurus sp. as Z3 and two from Monodelphis brevicaudata and Panstrongylus geniculatus as Z3 with a Z1 ASAT character. The ways in which stocks were isolated and grown up in vitro did not influence isozyme profile. These results support earlier evidence from Pará State that Z2 is absent from the Amazon basin and that the distribution of T. cruzi zymodemes in this region is quite different to that in endemic areas on the south of the continent.
在从亚马孙州和朗多尼亚州收集的、来自117个不同个体宿主的151株克氏锥虫样本中，来自113个宿主的147株被鉴定为酶型1（Z1）。这些样本包括来自三种有袋动物、两种啮齿动物和三种锥蝽的克氏锥虫样本，不过大多数样本来自普通负鼠（Didelphis marsupialis）。一株来自强壮红猎蝽（Rhodnius robustus）的克氏锥虫样本被鉴定为具有Z3磷酸葡萄糖变位酶（PGM）特征的Z1，一株来自松鼠属（Sciurus sp.）的样本为Z3，两株分别来自短尾袋鼬（Monodelphis brevicaudata）和膝状长红猎蝽（Panstrongylus geniculatus）的样本为具有Z1天冬氨酸氨基转移酶（ASAT）特征的Z3。样本的体外分离和培养方式不影响同工酶谱。这些结果支持了帕拉州早期的证据，即亚马孙盆地不存在Z2，且该地区克氏锥虫酶型的分布与该大陆南部的流行地区有很大不同。
