Devani M B, Shishoo C J, Patel M A, Bhalara D D
J Pharm Sci. 1978 May;67(5):661-3. doi: 10.1002/jps.2600670523.
A spectrophotometric determination of isoniazid in the presence of its hydrazones was developed. The method involves the reaction between isoniazid and 2,3-dichloro-1,4-naphthoquinone in the presence of ammonia in an ethanolic medium. The colored product has an absorbance maxium at 640 nm. The Lambert-Beer law is obeyed in the 1--14-microgram/ml range. The proposed method was applied to the analysis of isoniazid tablets. In commercial tablets, hydrazone formation due to the reaction between isoniazid and lactose was detected by TLC. The analysis of lactose-containing isoniazid tablets showed 10--22% lower recovery than that obtained by the official method. Hydrazone formation in tablets probably interferes with isoniazid bioavailability.
建立了一种在异烟肼腙存在下分光光度法测定异烟肼的方法。该方法涉及异烟肼与2,3 - 二氯 - 1,4 - 萘醌在乙醇介质中氨存在下的反应。有色产物在640 nm处有最大吸光度。在1 - 14微克/毫升范围内符合朗伯 - 比尔定律。该方法应用于异烟肼片剂的分析。在市售片剂中，通过薄层色谱法检测到由于异烟肼与乳糖反应形成了腙。含乳糖的异烟肼片剂分析显示回收率比法定方法低10 - 22%。片剂中腙的形成可能会干扰异烟肼的生物利用度。
