Davy C W, Jackson M R, Walker J M
Clin Chem. 1984 Jan;30(1):101-4.
The stability of various marmoset (Callithrix jacchus) plasma constituents was investigated after storage at room temperature, 4 degrees C, and -20 degrees C. The method of sequential analysis ensured that the between-run bias of the methods of analysis used was drastically reduced, and the definitions of stability were linked to the imprecision of these methods. Optimal conditions for storage for as long as 48 h depended on the analyte being measured. Room temperature was optimal for cholinesterase and acetylcholinesterase; 4 degrees C for protein, albumin, alanine aminotransferase, isocitrate dehydrogenase, sorbitol dehydrogenase, lactate dehydrogenase, and glutamate dehydrogenase; and -20 degrees C for glutathione reductase and alkaline phosphatase. For aspartate amino-transferase and gamma-glutamyltransferase, either 4 degrees C or -20 degrees C would be suitable. Reasons are advanced for some conflicting reports in the published work, and we emphasize the need to investigate each analyte and species separately.
研究了普通狨猴（Callithrix jacchus）血浆中各种成分在室温、4℃和-20℃储存后的稳定性。序贯分析方法确保了所使用分析方法的批间偏差大幅降低，并且稳定性的定义与这些方法的不精密度相关联。长达48小时的最佳储存条件取决于所测量的分析物。室温是胆碱酯酶和乙酰胆碱酯酶的最佳储存温度；4℃适合蛋白质、白蛋白、丙氨酸氨基转移酶、异柠檬酸脱氢酶、山梨醇脱氢酶、乳酸脱氢酶和谷氨酸脱氢酶；-20℃适合谷胱甘肽还原酶和碱性磷酸酶。对于天冬氨酸氨基转移酶和γ-谷氨酰转移酶，4℃或-20℃都合适。文中对已发表研究中一些相互矛盾的报告给出了原因，并且我们强调需要分别对每种分析物和物种进行研究。
