Duermeyer W, Stute R, Hellings J A
Dev Biol Stand. 1983;54:475-82.
An enzyme-linked immunosorbent assay (ELISA) was developed, based on sera from patients convalescent from non-A, non-B hepatitis and haemophilia A patients. Using this ELISA an antigen (DS-Ag) was detected which appears to be related to non-A, non-B hepatitis. The serum of another patient with haemophilia A, which was strongly positive for the DS-Ag, caused non-A, non-B hepatitis in a chimpanzee. DS-Ag could be detected in the serum of the chimpanzee during the acute phase of the infection. The ELISA for DS-Ag did not react with either hepatitis A or B virus antigens, or with Epstein-Barr virus or Cytomegalovirus. The assay was provisionally evaluated using sera from different groups of patients. From 17 patients with post-transfusion hepatitis non-A, non-B, 11 were found positive in the ELISA for DS-Ag (65%). A relatively high prevalence of DS-Ag (17%) and -antibodies (20%) was found in patients with haemophilia, who are regularly treated with blood clotting factor concentrates. The DS-Ag was found in 8 of 1400 (0,6%) volunteer blood donors, and antibody to DS-Ag in 3% of the sera. Remarkably, a high incidence (41%) of antibodies to DS-Ag was found in prostitutes, suggesting that this antigen may be transmitted by a sexual route as well. The DS-Ag was pelleted by ultracentrifugation for 4 h at 100.000 xg and was found to have a buoyant density of 1.32 g/cm3 in a CsCl gradient.
基于非甲非乙型肝炎康复患者和甲型血友病患者的血清，开发了一种酶联免疫吸附测定法（ELISA）。利用这种ELISA检测到一种抗原（DS-Ag），它似乎与非甲非乙型肝炎有关。另一名甲型血友病患者的血清对DS-Ag呈强阳性，该血清在一只黑猩猩身上引发了非甲非乙型肝炎。在感染急性期，可在黑猩猩的血清中检测到DS-Ag。针对DS-Ag的ELISA与甲型或乙型肝炎病毒抗原、爱泼斯坦-巴尔病毒或巨细胞病毒均无反应。使用不同患者组的血清对该测定法进行了初步评估。在17例输血后非甲非乙型肝炎患者中，11例在DS-Ag的ELISA检测中呈阳性（65%）。在定期接受凝血因子浓缩物治疗的血友病患者中，发现DS-Ag（17%）和抗体（20%）的患病率相对较高。在1400名志愿献血者中，有8人（0.6%）检测到DS-Ag，3%的血清中检测到DS-Ag抗体。值得注意的是，在妓女中发现DS-Ag抗体的发生率很高（41%），这表明该抗原也可能通过性途径传播。DS-Ag在100000×g下超速离心4小时后形成沉淀，在氯化铯梯度中其浮密度为1.32 g/cm³。
