Joenje H, Oostra A B
Hum Genet. 1983;65(2):99-101. doi: 10.1007/BF00286642.
Blood samples from four healthy individuals and from seven Fanconi anaemia (FA) patients were cultured at oxygen tensions ranging from 3% to 45% O2. Cultures were harvested at 72 h and scored for chromosomal aberrations. In the majority of FA patients the aberration frequency showed a tendency to increase as a function of oxygen tension over the culture, whereas the aberration frequency in healthy individuals was not affected. However, the response in FA cultures was variable among patients and in individual cases when assayed on different occasions. A much stronger effect of oxygen tension was observed when the FA blood samples had been treated with mitomycin C (0.25 microgram/ml, 30 min) before culture initiation.
从4名健康个体和7名范可尼贫血（FA）患者采集的血样在氧气张力范围为3%至45% O₂ 的条件下进行培养。培养72小时后收获细胞培养物，并对染色体畸变进行评分。在大多数FA患者中，畸变频率呈现出随着培养过程中氧气张力的升高而增加的趋势，而健康个体的畸变频率则不受影响。然而，FA细胞培养物的反应在不同患者之间以及在不同时间对个体病例进行检测时存在差异。当FA血样在培养开始前用丝裂霉素C（0.25微克/毫升，30分钟）处理时，观察到氧气张力的影响要强得多。
