Stoica G H, Balea M, Michiu V
Med Interne. 1983 Oct-Dec;21(4):307-13.
By exploiting the polymer-enhancing effect on the immunological precipitation, we have developed a highly sensitive, single radial-immunodiffusion (SRID) method for measuring albumin in urine. As little as 20 ng of albumin could be measured in a 4-microL sample of urine. Both within- and between-plate coefficients of variation were less than 5% over the range of albumin concentrations between 5 and 80 mg/L. Accuracy, as estimated from recovery studies, was also very good. The method is simple to perform and inexpensive (it requires only very small amounts of antiserum). The urinary albumin output, as determined on timed urine collections from 117 healthy persons (66 women and 51 men), ranged from 1.8 to 17.7 micrograms/min, with a geometric mean value of 6.8 micrograms/min (log SD = 0.1894). Based on the above-mentioned advantages of the method, we recommend it for routine use in clinical laboratories.
通过利用聚合物对免疫沉淀的增强作用，我们开发了一种用于测量尿白蛋白的高灵敏度单向放射免疫扩散（SRID）方法。在4微升尿样中，可检测到低至20纳克的白蛋白。在白蛋白浓度为5至80毫克/升的范围内，板内和板间变异系数均小于5%。根据回收率研究估计，准确性也非常好。该方法操作简单且成本低廉（仅需极少量抗血清）。对117名健康人（66名女性和51名男性）定时收集的尿液进行测定，尿白蛋白排出量在1.8至17.7微克/分钟之间，几何平均值为6.8微克/分钟（对数标准差=0.1894）。基于该方法的上述优点，我们推荐其在临床实验室中常规使用。
