Exner T, Rickard K A, Kronenberg H
Thromb Res. 1983 Nov 15;32(4):427-36. doi: 10.1016/0049-3848(83)90094-4.
Samples of normal tissues taken from three patients within 24 hours of death were physically disrupted, washed and extracted. Centrifuged supernatants were tested for factor VIII coagulant antigen (VIII CAg) and factor VIII related antigen (VIII RAg) by radioimmunoassays using homologous and heterologous antisera respectively. VIII RAg was readily washed out by isotonic saline whereas VIII CAg required 1.5 M sodium chloride for optimal extraction from tissue homogenates. Highest levels of VIII CAg were detected in some lymph nodes followed by lung, liver and spleen. There was rapid loss of VIII CAg from most tissues in the absence of proteolytic inhibitors.
在三名患者死亡后24小时内采集的正常组织样本进行了物理破碎、洗涤和提取。分别使用同源和异源抗血清通过放射免疫测定法对离心后的上清液进行因子VIII凝血抗原（VIII CAg）和因子VIII相关抗原（VIII RAg）检测。VIII RAg很容易被等渗盐水洗去，而VIII CAg则需要1.5M氯化钠才能从组织匀浆中进行最佳提取。在一些淋巴结中检测到最高水平的VIII CAg，其次是肺、肝和脾。在没有蛋白水解抑制剂的情况下，大多数组织中的VIII CAg迅速丧失。
