凝血因子VIII抗原在豚鼠组织及分离的肝细胞组分中的定位

Localization of factor VIIIC: antigen in guinea-pig tissues and isolated liver cell fractions.

作者信息

Kelly D A, Summerfield J A, Tuddenham E G

出版信息

Br J Haematol. 1984 Apr;56(4):535-43. doi: 10.1111/j.1365-2141.1984.tb02178.x.

DOI:10.1111/j.1365-2141.1984.tb02178.x

Abstract

Factor VIIIC:antigen (VIII:CAg) was estimated in guinea-pig tissues by an immunoradiometric assay using a human inhibitor antibody. In homogenized guinea-pig tissues, VIII:CAg was shown to be stable and to be predominantly located in the liver (9 +/- 1.2 units; mean +/- SEM, n = 8). Lesser amounts were detected in spleen (1.3 +/- 0.02 units), lung (0.6 +/- 0.07) and kidney (0.4 +/- 0.06). In isolated liver cell fractions separated by centrifugal elutriation VIII:CAg was mainly detected in the hepatocyte fraction (0.3 +/- 0.07 units/10(8) cells;mean +/- SEM, n = 5) and in lesser amounts in the endothelial (0.02 +/- 0.01 units/10(8) cells) and the Kupffer cell fractions (0.05 +/- 0.02 units/10(8) cells). The liver concentration of VIII:CAg was (0.17 +/- 0.02 units/g) which was 20% of the plasma concentration (0.96 +/- 0.01 units/ml, n = 8) suggesting that VIII:CAg may not be stored in the liver but is rapidly exported following synthesis.

摘要

使用人抑制剂抗体通过免疫放射分析测定豚鼠组织中的凝血因子VIII抗原（VIII:CAg）。在匀浆的豚鼠组织中，VIII:CAg显示稳定，且主要位于肝脏（9±1.2单位；平均值±标准误，n = 8）。在脾脏（1.3±0.02单位）、肺（0.6±0.07）和肾脏（0.4±0.06）中检测到的量较少。在通过离心淘析分离的分离肝细胞组分中，VIII:CAg主要在肝细胞组分中检测到（0.3±0.07单位/10⁸个细胞；平均值±标准误，n = 5），在内皮细胞组分（0.02±0.01单位/10⁸个细胞）和库普弗细胞组分（0.05±0.02单位/10⁸个细胞）中检测到的量较少。肝脏中VIII:CAg的浓度为（0.17±0.02单位/克），为血浆浓度（0.96±0.01单位/毫升，n = 8）的20%，这表明VIII:CAg可能不是储存在肝脏中，而是在合成后迅速输出。