在相同植物组织样本中同时测定叶绿素a和叶绿素b以及14CO2固定量。
The determination of chlorophylls a and b together with 14CO2 fixation in the same plant tissue samples.
作者信息
Lewinsohn E, Gressel J
出版信息
Anal Biochem. 1983 Dec;135(2):438-42. doi: 10.1016/0003-2697(83)90708-x.
PMID:6419638
Abstract
A simple, efficient, and inexpensive method for measuring radioactivity as well as chlorophylls a and b in a large number of plant tissue samples is presented. Chlorophyll is determined following extraction with dimethyl sulfoxide or N-N'-dimethylformamide. The solvent is then evaporated on glass-fiber filters and bleached under light. The filter disks are counted together with the cleared plant material.
摘要
本文介绍了一种简单、高效且经济的方法，用于测量大量植物组织样本中的放射性以及叶绿素a和叶绿素b。叶绿素通过用二甲基亚砜或N-N'-二甲基甲酰胺提取后进行测定。然后将溶剂在玻璃纤维滤纸上蒸发，并在光照下漂白。将滤纸片与清理后的植物材料一起计数。
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