Zierold K, Schäfer D
J Microsc. 1978 Jan;112(1):89-93. doi: 10.1111/j.1365-2818.1978.tb01156.x.
X-ray microanalysis in the scanning electron microscope (SEM) is used to obtain information about the distribution and mobility of electrolyte ions in the skeletal muscle tissue. In order to localize the diffusible ions in their physiological state, so far as possible, any chemical fixative is avoided. A special 'cooling chain' preparation method is applied which enables studies on cryofractured bulk specimens at low temperatures. Quantitation of the obtained X-ray spectra is done by the use of glycerol-treated tissue specimens which are incubated with electrolyte solutions of defined ion concentration.
扫描电子显微镜（SEM）中的X射线微分析用于获取有关骨骼肌组织中电解质离子分布和迁移率的信息。为了尽可能在生理状态下定位可扩散离子，要避免使用任何化学固定剂。采用一种特殊的“冷却链”制备方法，该方法能够在低温下对冷冻断裂的大块标本进行研究。通过使用甘油处理过的组织标本进行获得的X射线光谱的定量分析，这些标本与具有确定离子浓度的电解质溶液一起孵育。
