Zierold K, Pietruschka F, Schäfer D
Microsc Acta. 1979 May;81(5):361-6.
Skeletal muscle in culture is used to demonstrate that the intracellular concentration of elements in tissue culture cells, especially of diffusible ions such as sodium, chlorine, potassium, and calcium can be measured by X-ray microanalysis in the scanning electron microscope. Quantitative analysis is possible by comparison with X-ray spectra of cells incubated in electrolyte solutions of known concentration. The minimum detectable concentration is approximately 2 mM.
培养中的骨骼肌用于证明组织培养细胞中元素的细胞内浓度，特别是诸如钠、氯、钾和钙等可扩散离子的浓度，可以通过扫描电子显微镜中的X射线微分析来测量。通过与在已知浓度电解质溶液中孵育的细胞的X射线光谱进行比较，可以进行定量分析。最低可检测浓度约为2 mM。
