Tsuboi S, Nakagawa T, Tomita M, Seo T, Ono H, Kawamura K, Iwamura N
Cancer Res. 1984 Mar;44(3):1231-4.
Aflatoxin B1 (AFB1) was detected in serum samples of healthy Japanese males by radioimmunoassay and high-performance liquid chromatography. Blood samples were obtained from 20 subjects after fasting and from 80 subjects after lunch. The subjects ranged in age from 20 to 63 years of age. Measurement of AFB1 in the samples was performed by radioimmunoassay and was confirmed by high-performance liquid chromatography using a mu Porasil column and a C18-mu Bondapak column after the conversion of AFB1 to its water adduct AFB2a. AFB1 was detected in 5 of 20 fasting blood samples [20 to 56 pg/ml of serum; 33.6 +/- 14.6 (S.D.)] and in 29 of 80 serum samples taken after lunch (20 to 1169 pg/ml of serum; 218.1 +/- 268.3). Mass spectral analysis of the material obtained after high-performance liquid chromatography separation from serum samples confirmed the presence of AFB1.
通过放射免疫分析法和高效液相色谱法在健康日本男性的血清样本中检测到黄曲霉毒素B1（AFB1）。在禁食后从20名受试者采集血样，午餐后从80名受试者采集血样。受试者年龄在20至63岁之间。样本中AFB1的测量通过放射免疫分析法进行，并在将AFB1转化为其水加合物AFB2a后，使用μPorasil柱和C18-μBondapak柱通过高效液相色谱法进行确认。在20份禁食血样中的5份中检测到AFB1[血清中为20至56 pg/ml；33.6±14.6（标准差）]，在午餐后采集的80份血清样本中的29份中检测到AFB1（血清中为20至1169 pg/ml；218.1±268.3）。对血清样本进行高效液相色谱分离后获得的物质进行质谱分析，证实了AFB1的存在。
