采用酶联免疫吸附测定法和高效液相色谱法检测进口到日本的食品中的黄曲霉毒素B1。

Detection of aflatoxin B1 in imported food products into Japan by enzyme-linked immunosorbent assay and high performance liquid chromatography.

作者信息

Adachi Y, Hara M, Kumazawa N H, Hirano K, Ueno I, Egawa K

机构信息

National Institute of Animal Health, Ibaraki, Japan.

出版信息

J Vet Med Sci. 1991 Feb;53(1):49-52. doi: 10.1292/jvms.53.49.

DOI:10.1292/jvms.53.49

PMID:1830779

Abstract

In order to detect the presence of aflatoxin B1 (AFB1), the use of the enzyme-linked immunosorbent assay (ELISA) and recovery test was evaluated. The detection limit of ELISA for AFB1 was 1 pg/assay and the recovery from maize spiked with AFB1 exceeded 80%. AFB1 was detected by ELISA in seven out of twelve samples of imported food products including peanut, almond, red pepper, cocoa bean, black pepper, buckwheat, walnut, adlay, soybean, popcorn, and pistachio nut, and by high performance liquid chromatography (HPLC) in four of the samples. However, the content of AFB1 in these samples was less than 10 ng/g of the minimum value authorized by the Japanese sanitation law. These results demonstrate that ELISA is more sensitive than HPLC and imported food products are broadly contaminated with AFB1.

摘要

为了检测黄曲霉毒素B1（AFB1）的存在，对酶联免疫吸附测定法（ELISA）和回收率测试的使用进行了评估。ELISA对AFB1的检测限为1 pg/测定，从添加了AFB1的玉米中的回收率超过80%。在包括花生、杏仁、红辣椒、可可豆、黑胡椒、荞麦、核桃、薏仁、大豆、爆米花和开心果在内的12种进口食品样品中，有7种通过ELISA检测出AFB1，4种通过高效液相色谱法（HPLC）检测出AFB1。然而，这些样品中AFB1的含量低于日本卫生法规定的最低值10 ng/g。这些结果表明，ELISA比HPLC更灵敏，并且进口食品广泛受到AFB1的污染。