Ratcliffe M J
Cell Immunol. 1984 Jan;83(1):208-14. doi: 10.1016/0008-8749(84)90240-5.
Chronic allotype suppression was generated in M-1 (C mu), G-1 (C gamma) heterozygous B14 line chickens either by embryonal injection or by maternal transfer of anti-M-1b allotype antibodies. Using a sensitive radioimmunoassay to detect the suppressed IgM-1b, a striking disparity was observed between the degree of suppression of the IgM-1b allotype and that of the genetically linked IgG-1i allotype. The amount of suppressed IgM-1b ranged between 0.2 and 3% of total serum IgM in chronically suppressed birds. The levels of the genetically linked IgG-1i, however, comprised 5 to 25% of total serum IgG in such birds. The allotypes measured in suppressed chickens were qualitatively identical to those in normal, nonsuppressed birds by serological criteria. These results are discussed within the context of isotype regulation in the chicken.
通过胚胎注射或抗M-1b同种异型抗体的母体转移，在M-1（Cμ）、G-1（Cγ）杂合B14系鸡中产生了慢性同种异型抑制。使用灵敏的放射免疫测定法检测受抑制的IgM-1b，发现IgM-1b同种异型的抑制程度与基因连锁的IgG-1i同种异型的抑制程度之间存在显著差异。在慢性受抑制的鸡中，受抑制的IgM-1b量占血清总IgM的0.2%至3%。然而，在这些鸡中，基因连锁的IgG-1i水平占血清总IgG的5%至25%。根据血清学标准，受抑制鸡中检测到的同种异型与正常未受抑制鸡中的同种异型在质量上是相同的。在鸡的同种型调节背景下讨论了这些结果。
