Järvinen M, Pernu H, Rinne A, Hopsu-Havu V K, Altonen M
Acta Histochem. 1983;73(2):279-82. doi: 10.1016/S0065-1281(83)80039-7.
3 known human cysteineproteinase inhibitors--neutral cysteineproteinase inhibitor (NCPI), acid cysteineproteinase inhibitor (ACPI) and serum alpha-cysteineproteinase inhibitor (alpha-CPI)--were localized immunohistochemically in the squamous epithelium of the mouth by using the peroxidase-antiperoxidase complex method and formaldehyde-fixed paraffin-embedded tissue sections. Neither ACPI nor NCPI was found in the 2 to 3 most basal cell layers of the epithelium, but a strong cytoplasmic reaction was seen in the upper cell layers by using either anti-ACPI or anti-NCPI serum. In some cells the most dense stain precipitate was seen around or in the nucleus, and in the highest cell layers the staining was sometimes associated with the cell membrane. alpha-CPI was localized mainly in the epithelial stroma and in the intercellular space of the epithelium.
采用过氧化物酶 - 抗过氧化物酶复合物法及甲醛固定石蜡包埋组织切片，对3种已知的人半胱氨酸蛋白酶抑制剂——中性半胱氨酸蛋白酶抑制剂（NCPI）、酸性半胱氨酸蛋白酶抑制剂（ACPI）和血清α - 半胱氨酸蛋白酶抑制剂（α - CPI）进行免疫组织化学定位，观察其在口腔鳞状上皮中的分布。上皮最基底的2至3层细胞中未发现ACPI和NCPI，但使用抗ACPI或抗NCPI血清时，在上层细胞中可见强烈的细胞质反应。在一些细胞中，最密集的染色沉淀物见于细胞核周围或核内，在最高层细胞中，染色有时与细胞膜相关。α - CPI主要定位于上皮基质和上皮细胞间隙。
