采用枯草芽孢杆菌重组分析和沙门氏菌/微粒体回复突变分析对生药进行致突变性筛选。
Mutagenicity screening of crude drugs with Bacillus subtilis rec-assay and Salmonella/microsome reversion assay.
作者信息
Morimoto I, Watanabe F, Osawa T, Okitsu T, Kada T
出版信息
Mutat Res. 1982 Apr;97(2):81-102. doi: 10.1016/0165-1161(82)90007-3.
This paper describes the screening studies of 104 commercial crude drugs for mutagenicity by the rec-assay with Bacillus subtilis as well as the reversion assay with Ames strains TA98 and TA100 of Salmonella typhimurium. The rec-assays showed that 13 water extracts and 27 methanol extracts of the crude drugs were positive. The Ames assays with or without metabolic activation showed that 24 water extracts and 16 methanol extracts were mutagenic. In total, mutagenic activities were found in 45 samples among the 104 crude drugs tested.
本文描述了采用枯草芽孢杆菌rec - 检测法以及鼠伤寒沙门氏菌Ames菌株TA98和TA100的回复突变检测法，对104种市售粗提药物进行致突变性筛选研究的情况。rec - 检测显示，这些粗提药物的13种水提取物和27种甲醇提取物呈阳性。有或无代谢活化的Ames检测表明，24种水提取物和16种甲醇提取物具有致突变性。在所检测的104种粗提药物中，总共在45个样品中发现了致突变活性。
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