Moreno E, Jones L M, Berman D T
Infect Immun. 1984 Mar;43(3):779-82. doi: 10.1128/iai.43.3.779-782.1984.
Lipopolysaccharides (LPS) were extracted from rough strains of Brucella abortus and Brucella melitensis and from strains of the naturally occurring rough species Brucella ovis and Brucella canis. Brucella rough lipopolysaccharides (R-LPS) were readily distinguished from Brucella smooth lipopolysaccharides (S-LPS) and enterobacterial R-LPS, by their chemical, physical, and serological characteristics. B. ovis R-LPS was differentiated from B. abortus, B. melitensis, and B. canis R-LPS by its reaction of partial identity in immunodiffusion. Monospecific mouse sera against B. ovis R-LPS agglutinated only the homologous bacteria but not R cells of other species of Brucella. B. ovis R-LPS contained more 2-keto, 3-deoxyoctonate, and glucosamine as a percentage of dry weight than any other R-LPS tested. B. abortus R-LPS was identified by the absence of an unidentified sugar present in the other R-LPS molecules, and B. melitensis R-LPS could be differentiated from B. canis R-LPS by its higher content of fatty acids. In contrast to S-LPS, all of the R-LPS studied lacked quinovosamine. In electron micrographs, Brucella R-LPS had a granular appearance, in contrast to typical lamellar structures formed by Brucella S-LPS and Escherichia coli R-LPS.
脂多糖（LPS）从流产布鲁氏菌和羊种布鲁氏菌的粗糙菌株以及自然存在的粗糙种绵羊布鲁氏菌和犬布鲁氏菌的菌株中提取。通过其化学、物理和血清学特征，布鲁氏菌粗糙脂多糖（R-LPS）很容易与布鲁氏菌光滑脂多糖（S-LPS）和肠杆菌R-LPS区分开来。绵羊布鲁氏菌R-LPS在免疫扩散中通过其部分相同反应与流产布鲁氏菌、羊种布鲁氏菌和犬布鲁氏菌R-LPS区分开来。针对绵羊布鲁氏菌R-LPS的单特异性小鼠血清仅凝集同源细菌，而不凝集其他布鲁氏菌种的R细胞。与所测试的任何其他R-LPS相比，绵羊布鲁氏菌R-LPS干重中2-酮基-3-脱氧辛酸和葡糖胺的含量更高。流产布鲁氏菌R-LPS通过其他R-LPS分子中不存在一种未鉴定的糖而得以鉴定，羊种布鲁氏菌R-LPS可通过其较高的脂肪酸含量与犬布鲁氏菌R-LPS区分开来。与S-LPS不同，所有研究的R-LPS都缺乏奎诺糖胺。在电子显微镜照片中，布鲁氏菌R-LPS呈颗粒状外观，这与布鲁氏菌S-LPS和大肠杆菌R-LPS形成的典型层状结构不同。
