Moreno E, Speth S L, Jones L M, Berman D T
Infect Immun. 1981 Jan;31(1):214-22. doi: 10.1128/iai.31.1.214-222.1981.
Purified lipopolysaccharide (LPS) extracted with phenol-water from smooth Brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees C. The degraded polysaccharide (AH) released gave reactions of identity with the native polysaccharide hapten (NH) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of B. abortus and with the polysaccharide (poly B) extracted by trichloroacetic acid from rough B. melitensis strain B115. Poly B was present in the soluble cytoplasmic fraction but not in the membrane fraction, of disrupted B115 cells. It could not be extracted from three rough mutants of B. abortus or from B canis or B. ovis cells. Both AH and NH shared determinants present on smooth LPS and missing from poly B. Sugars found in purified LPS, NH, and AH included mannose, glucose, quinovosamine, glucosamine, and 2-keto-3-deoxyoctonate. Poly B contained only a trace amount of quinovosamine and no 2-keto-3-deoxyoctonate detectable by the thiobarbiturate assay. Sera from some rabbits immunized with pure smooth LPS and some, but not all, cows infected with field strains of B. abortus recognized the determinants missing from poly B. A subclass-specific enzyme-linked immunoassay showed that most of the antibody in sera from infected cows which binds to smooth LPS and to NH is of the immunoglobulin G1 subclass.
用酚水法从光滑型流产布鲁氏菌中提取的纯化脂多糖（LPS）在100℃下用1%乙酸水解。释放出的降解多糖（AH）与流产布鲁氏菌酚水或三氯乙酸提取的内毒素制剂中的天然多糖半抗原（NH）以及从粗糙型羊种布鲁氏菌B115菌株中用三氯乙酸提取的多糖（聚B）具有相同反应。聚B存在于破碎的B115细胞的可溶性细胞质部分，而不存在于膜部分。它不能从流产布鲁氏菌的三个粗糙突变体或犬布鲁氏菌或绵羊布鲁氏菌细胞中提取。AH和NH都具有光滑型LPS上存在而聚B中缺失的决定簇。纯化的LPS、NH和AH中发现的糖类包括甘露糖、葡萄糖、奎诺糖胺、氨基葡萄糖和2-酮-3-脱氧辛酸。聚B仅含有痕量的奎诺糖胺，用硫代巴比妥酸测定法未检测到2-酮-3-脱氧辛酸。一些用纯光滑型LPS免疫的兔血清以及一些（但不是全部）感染流产布鲁氏菌野毒株的奶牛血清识别聚B中缺失的决定簇。一种亚类特异性酶联免疫测定表明，感染奶牛血清中与光滑型LPS和NH结合的大多数抗体属于免疫球蛋白G1亚类。
