George R B, Lambert R S
South Med J. 1984 Feb;77(2):161-3. doi: 10.1097/00007611-198402000-00006.
To evaluate the specificity of serologic tests for histoplasmosis in an endemic area, we studied sera from 104 consecutive healthy blood donors, testing for antibodies to either histoplasmin or Histoplasma yeast antigen, using complement fixation (CF), radioimmunoassay (RIA), and radial immunodiffusion (ID). Twenty-five subjects (24%) had CF antibody titers of 1:8 or 1:16 to one or both antigens; none had titers above 1:16. Nine subjects (9%) had RIA antibody titers of 1:8 or higher. No titers above 1:8 were found using the yeast antigen; five subjects had titers of greater than 1:16 to histoplasmin using RIA. No precipitin bands were found in any of these subjects. The results of four previous studies in endemic populations yielded similar or lower incidences of positive tests. CF antibody titers to either antigen, RIA antibody titers to yeast antigen of 1:32 or greater, or precipitin bands are rare in persons who live where histoplasmosis is endemic.
为评估在地方性流行区组织胞浆菌病血清学检测的特异性，我们研究了104名连续健康献血者的血清，采用补体结合试验（CF）、放射免疫测定（RIA）和单向免疫扩散法（ID）检测针对组织胞浆菌素或组织胞浆菌酵母抗原的抗体。25名受试者（24%）针对一种或两种抗原的CF抗体效价为1:8或1:16；无人抗体效价高于1:16。9名受试者（9%）的RIA抗体效价为1:8或更高。使用酵母抗原未发现抗体效价高于1:8；5名受试者使用RIA检测针对组织胞浆菌素的抗体效价大于1:16。这些受试者均未发现沉淀带。之前在地方性流行人群中进行的四项研究结果显示，检测呈阳性的发生率相似或更低。在组织胞浆菌病地方性流行地区生活的人群中，针对任何一种抗原的CF抗体效价、针对酵母抗原的RIA抗体效价达到1:32或更高，或出现沉淀带的情况都很罕见。
