[不同波长紫外线照射后人红细胞表面的功能和结构变化。I. ABO和恒河猴血型系统抗原的表达]
[Functional and structural changes in the surface of human erythrocytes after irradiation with UV rays of various wave lengths. I. The expression of ABO and rhesus system antigens].
作者信息
Samoĭlova K A, Klimova K N, Priezzheva L S, Arstishevskaia R A
出版信息
Tsitologiia. 1983 Dec;25(12):1378-86.
UV irradiation (254 nm) in doses increasing erythrocyte haemolysis by 5, 10, 18 and 28 per cent was found to stimulate, by 2--16 times, the agglutination activity of ABO and Rh system antigens. The stimulation effect was the higher the lower the antigen activity before irradiation. In the Rh-negative (Rh-) erythrocytes, irradiation induced manifestation of the Rh0(D)-antigen specific activity suggesting that this antigen may be present in the Rh- erythrocyte membrane. The expression of Rh0(D)-antigen in Rh- erythrocytes, the stimulation of its activity in Rh-positive cells, and the activation of ABO system antigens may result from a photochemical destruction of the outer perimembraneous layer and release some of its components which stain in situ with alcian blue to be presumably glycoproteins. This effect is necessary to keep in mind when UV-irradiated blood transfusion is performed in therapeutic aims Rh- patients.
研究发现，紫外线（254纳米）照射剂量可使红细胞溶血率分别提高5%、10%、18%和28%，其可将ABO和Rh系统抗原的凝集活性提高2至16倍。照射前抗原活性越低，刺激效果越明显。在Rh阴性（Rh-）红细胞中，照射可诱导Rh0（D）抗原特异性活性的表现，这表明该抗原可能存在于Rh-红细胞膜中。Rh-红细胞中Rh0（D）抗原的表达、Rh阳性细胞中其活性的刺激以及ABO系统抗原的激活，可能是由于膜外周外层的光化学破坏并释放了一些成分，这些成分经阿尔辛蓝原位染色后推测为糖蛋白。在对Rh-患者进行紫外线照射血液输血以达到治疗目的时，必须牢记这一效应。
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