Fain J N, Li S Y, Litosch I, Wallace M
Biochem Biophys Res Commun. 1984 Feb 29;119(1):88-94. doi: 10.1016/0006-291x(84)91622-x.
The combination of 1.6 microM 4 beta phorbol, 12 beta myristate, 13 alpha acetate (PMA) and 1 microM A23187 produced a five-fold greater stimulation of rat hepatocyte glycogen phosphorylase activity than was seen with PMA alone. Vasopressin activation of glycogen phosphorylase was comparable to that seen with PMA plus A23187. Glycogen phosphorylase activity due to PMA plus A23187 was increased significantly after 30 sec, maximal at 120 and sustained at elevated levels for 240 sec. In contrast, activation due to vasopressin was maximal at 30 sec followed by a decrease. The addition of PMA 5 min prior to the A23187 abolished the synergism between these two agents. These data are compatible with the hypothesis that diacylglycerol and Ca2+ synergistically increase glycogen phosphorylase activity in rat hepatocytes.
1.6微摩尔4β佛波醇、12β肉豆蔻酸酯、13α乙酸酯（佛波酯，PMA）与1微摩尔A23187联合使用时，对大鼠肝细胞糖原磷酸化酶活性的刺激作用比单独使用PMA时强五倍。血管加压素对糖原磷酸化酶的激活作用与PMA加A23187时相当。PMA加A23187导致的糖原磷酸化酶活性在30秒后显著增加，120秒时达到最大值，并在升高水平维持240秒。相比之下，血管加压素引起的激活在30秒时达到最大值，随后下降。在加入A23187前5分钟加入PMA可消除这两种药物之间的协同作用。这些数据与二酰基甘油和Ca2 +协同增加大鼠肝细胞糖原磷酸化酶活性的假说相符。
