Kearns R J, Leu R W
Cell Immunol. 1984 Apr 1;84(2):361-71. doi: 10.1016/0008-8749(84)90108-4.
Mice that received a sublethal, intraperitoneal dose of viable Listeria monocytogenes, virulent strain 10403, exhibited a systemic increase in natural killer (NK) activity. The kinetics of the response differed with respect to the various effector cell populations analyzed. Resident peritoneal cells and peripheral blood leukocytes demonstrated high NK activity on Days 3, 7, and 10. Peak spleen and bone marrow NK activity was observed on Day 3, returning to normal levels by Day 7. In contrast, peritoneal exudate cells, elicited with proteose peptone, expressed enhanced NK activity for 60 days following infection with viable Listeria. Augmented NK activity was detected with all cell types as early as 12 hr after infection. The intraperitoneal injection of nonviable antigenic preparations derived from L. monocytogenes, strain 10403, resulted in the enhancement of peritoneal and splenic NK activity. In contrast, mice that received an intraperitoneal injection of avirulent Listeria, strain 19113, failed to express enhanced levels of NK activity. The genetic trait of anti-listerial resistance which is associated with non-H-2 linked genes was of no importance with respect to enhanced NK activity. Listeria-resistant C57BL/6J and Listeria-susceptible DBA/2J mice both produced systemic augmentation of NK activity following infection. NK activity was not abrogated by macrophage depletion or by treatment with anti-Thy 1.2 serum plus complement. These results confirm the potent immunostimulatory capacity of virulent Listeria for NK activity and provide further insight into the kinetics of this response in various lymphoid compartments. The protracted augmentation of NK activity of elicited peritoneal exudate cells as compared to nonelicited peritoneal cells in Listeria-primed mice suggests that the influx of inflammatory cells may provide NK-enriched and/or accessory populations for immunopotentiation of NK activity in inflammatory sites.
接受亚致死剂量、腹腔注射活的单核细胞增生李斯特菌强毒株10403的小鼠，其体内自然杀伤（NK）活性出现全身性增加。针对所分析的各种效应细胞群体，反应动力学有所不同。驻留腹腔细胞和外周血白细胞在第3、7和10天表现出高NK活性。脾脏和骨髓NK活性在第3天达到峰值，到第7天恢复到正常水平。相比之下，用蛋白胨诱导产生的腹腔渗出细胞，在感染活的李斯特菌后60天内均表现出增强的NK活性。早在感染后12小时，所有细胞类型均检测到NK活性增强。腹腔注射源自10403株单核细胞增生李斯特菌的无活性抗原制剂，可增强腹腔和脾脏的NK活性。相比之下，接受腹腔注射无毒李斯特菌19113株的小鼠，未能表现出NK活性增强。与非H-2连锁基因相关的抗李斯特菌抗性遗传特征，对于增强NK活性并不重要。抗李斯特菌的C57BL/6J小鼠和对李斯特菌敏感的DBA/2J小鼠在感染后均产生全身性NK活性增强。巨噬细胞耗竭或用抗Thy 1.2血清加补体处理均未消除NK活性。这些结果证实了强毒李斯特菌对NK活性具有强大的免疫刺激能力，并进一步深入了解了这种反应在各种淋巴组织中的动力学。与未诱导的腹腔细胞相比，在经李斯特菌致敏的小鼠中，诱导产生的腹腔渗出细胞的NK活性长期增强，这表明炎症细胞的流入可能为炎症部位NK活性的免疫增强提供富含NK的和/或辅助性群体。
