对Ektachem 400分析仪中二氧化碳电位测定法的干扰。

Interferences with potentiometry of CO2 in the Ektachem 400 Analyzer.

作者信息

Steelman M, Smith C H, Menon A, Thach B T, Hillman R E, Landt M

出版信息

Clin Chem. 1984 Apr;30(4):562-5.

Abstract

We have investigated the Kodak Ektachem 400 Analyzer procedure for CO2 for interferences from benzyl alcohol, benzoic acid, and several compounds structurally similar to benzoic acid. Benzoic acid in plasma, at concentrations found in neonates intoxicated with benzyl alcohol, caused a large increase in the results for CO2, as did substantially above-normal concentrations of certain fatty acids and keto-acids, and toxic concentrations of aspirin. We observed a correlation between increasing benzoic acid concentrations (up to 17 mmol/L) and falsely increasing CO2 values (greater than 47 mmol/L) obtained with the Ektachem Analyzer for samples from a neonate in the intensive-care unit, who was receiving benzyl alcohol-preserved saline solutions. Although the Ektachem CO2 procedure is simple and rapid, and in most cases accurate, questionable results are occasionally encountered, as indicated by a low anion gap or a measured CO2 exceeding that calculated from blood gas measurements. Such results require the use of another method for verification.

摘要

我们研究了柯达Ektachem 400分析仪测定二氧化碳的方法，以检测苯甲醇、苯甲酸以及几种结构与苯甲酸相似的化合物所产生的干扰。血浆中的苯甲酸，在因苯甲醇中毒的新生儿体内所发现的浓度下，会导致二氧化碳检测结果大幅升高，某些脂肪酸和酮酸的浓度大幅高于正常水平以及阿司匹林的中毒浓度时也会如此。我们观察到，对于重症监护病房中一名正在接受含苯甲醇保存的生理盐水溶液的新生儿的样本，随着苯甲酸浓度升高（高达17 mmol/L），用Ektachem分析仪测得的二氧化碳值会错误升高（大于47 mmol/L）。尽管Ektachem二氧化碳检测方法简单快速，且在大多数情况下准确，但偶尔也会遇到可疑结果，如阴离子间隙偏低或测得的二氧化碳超过血气测量计算所得的值。此类结果需要用另一种方法进行验证。