Julkunen I, Ukkonen P, Stenvik M, Hovi T, Renkonen L, Mäkelä O
J Clin Immunol. 1987 Jul;7(4):319-26. doi: 10.1007/BF00915554.
Immunoglobulin isotype composition of poliovirus antibodies was studied by isotype-specific solid-phase radio-immunoassay (RIA) in four patients with paralytic poliomyelitis, five adults receiving live poliovirus vaccine as a booster immunization, and seven children receiving first doses of inactivated poliovirus vaccine. In paralytic poliomyelitis serum and cerebrospinal fluid (CSF) poliovirus antibodies were mainly of IgG1, IgG3, and IgA isotypes. IgM antibodies were found in sera but not in CSF. Either IgG2 and IgG4 antibodies were undetectable or the titers were low. In adults who had received live trivalent poliovirus vaccine, antibodies against poliovirus type 3 were detected in IgG1 (53% of total antibodies), IgG3 (25%), IgM (9%), IgA (8%), IgG2 (3%), and IgG4 (2%) isotypes. In prevaccination and late postvaccination sera the share of IgG3 antibodies was exceptionally high (35%). In children who received inactivated poliovirus vaccine, antibodies developed in IgG1 (53-61% of total antibodies for poliovirus types 1, 2, and 3), IgG3 (12-21%), and IgM (23-33%) isotypes. Antibody levels in IgG2, IgG4, and IgA isotypes were low and observed only in a few cases. Like other viral antibodies IgG1 and IgG3 isotypes were the major IgG subclasses in poliovirus antibodies.
采用同型特异性固相放射免疫分析法（RIA），对4例麻痹型脊髓灰质炎患者、5例接受脊髓灰质炎活疫苗加强免疫的成人以及7例首次接种灭活脊髓灰质炎疫苗的儿童的脊髓灰质炎病毒抗体免疫球蛋白同型组成进行了研究。在麻痹型脊髓灰质炎患者中，血清和脑脊液（CSF）中的脊髓灰质炎病毒抗体主要为IgG1、IgG3和IgA同型。在血清中发现了IgM抗体，但脑脊液中未发现。要么检测不到IgG2和IgG4抗体，要么其滴度很低。在接受脊髓灰质炎活三价疫苗的成人中，针对3型脊髓灰质炎病毒的抗体在IgG1（占总抗体的53%）、IgG3（25%）、IgM（9%）、IgA（8%）、IgG2（3%）和IgG4（2%）同型中均有检测到。在接种疫苗前和接种疫苗后的晚期血清中，IgG3抗体的比例异常高（35%）。在接种灭活脊髓灰质炎疫苗的儿童中，抗体在IgG1（针对1、2和3型脊髓灰质炎病毒的总抗体的53 - 61%）、IgG3（12 - 21%）和IgM（23 - 33%）同型中产生。IgG2、IgG4和IgA同型中的抗体水平较低，仅在少数情况下观察到。与其他病毒抗体一样，IgG1和IgG3同型是脊髓灰质炎病毒抗体中的主要IgG亚类。
