Gorman S P, Scott E M, Hutchinson E P
J Appl Bacteriol. 1984 Feb;56(1):95-102. doi: 10.1111/j.1365-2672.1984.tb04699.x.
Bacillus subtilis spores with altered ionic content were tested for their susceptibility to lysis with lysozyme or sodium nitrite following treatment with glutaraldehyde. The Ca-form was more sensitive to glutaraldehyde (pH 4.0 and pH 7.9) than the untreated or H-form. Removal of spore coat dramatically increased sensitivity of the spore to glutaraldehyde. Pretreatment of spores, the coats of which had been extensively removed, with glutaraldehyde (pH 7.9) reduced the rate of lysis by lysozyme and by sodium nitrite, whereas glutaraldehyde at pH 4.0 had little effect. Glutaraldehyde pretreatment (pH 4.0 and pH 7.9) reduced the amount of hexosamine released by lysozyme but not by nitrite from isolated cortical fragments. Spore protoplasts were more susceptible to 0.01% (w/v) glutaraldehyde at pH 4.0 and isolated spore coats adsorbed alkaline glutaraldehyde more rapidly. These results are discussed in terms of a possible mode of action of glutaraldehyde on the bacterial spore.
对离子含量改变的枯草芽孢杆菌孢子进行了测试，以检测其在用戊二醛处理后对溶菌酶或亚硝酸钠裂解的敏感性。与未处理的或H型孢子相比，Ca型孢子对戊二醛（pH 4.0和pH 7.9）更敏感。去除芽孢衣显著增加了孢子对戊二醛的敏感性。用戊二醛（pH 7.9）对芽孢衣已大量去除的孢子进行预处理，可降低溶菌酶和亚硝酸钠引起的裂解速率，而pH 4.0的戊二醛几乎没有影响。戊二醛预处理（pH 4.0和pH 7.9）减少了溶菌酶从分离的芽孢皮层碎片中释放的己糖胺量，但未减少亚硝酸盐释放的己糖胺量。孢子原生质体在pH 4.0时对0.01%（w/v）戊二醛更敏感，分离的芽孢衣吸附碱性戊二醛更快。根据戊二醛对细菌孢子可能的作用方式对这些结果进行了讨论。
