Nakashima S, Kamikawa H, Ogita Z
J Biochem. 1983 Dec;94(6):1723-30. doi: 10.1093/oxfordjournals.jbchem.a134523.
In the IgG antibody response to bacterial alpha-amylase (B alpha A) assayed by the enzymatic procedure, C3H/He (C3) mice were high and C57BL/6 (B6) mice were low responders. High responsiveness was inherited as a dominant characteristic in (B6XC3)F1 hybrid mice. In these strains, the primary antibody response was analyzed for heterogeneity by isoelectric focusing (IEF). The IEF spectra were visualized with the use of the capacity of antibody to inhibit the amylase activity of antigen. Increases in the antigen dose and in the time interval between immunization and bleeding resulted in increases in antibody titers accompanied by strong staining of focused antibodies and by the expansion of the pH range where antibodies were focused. High responsiveness in C3 and F1 hybrid mice was also associated with the increase in intensity of stain and the rapid expansion of pH range of focused antibodies. Another strain difference was noted in the isoelectric point (pI) values of antibodies taken early in the primary response. B6 antisera contained those fractions of antibodies focusing over a more alkaline area than C3 antibodies. A similar strain difference in the pI values of antibodies occurred in the response to an irrelevant antigen, Taka-amylase A (TAA), suggesting that the hypervariable regions of antibody molecules play no major part in the strain difference observed. Antisera from F1 hybrid mice displayed bands covering the combined pH ranges of B6 and C3 spectrotypes.
在用酶促方法检测的对细菌α淀粉酶（BαA）的IgG抗体应答中，C3H/He（C3）小鼠是高应答者，而C57BL/6（B6）小鼠是低应答者。高应答性作为显性特征在（B6×C3）F1杂交小鼠中遗传。在这些品系中，通过等电聚焦（IEF）分析初次抗体应答的异质性。利用抗体抑制抗原淀粉酶活性的能力使IEF图谱可视化。抗原剂量的增加以及免疫与采血之间时间间隔的延长导致抗体滴度增加，同时聚焦抗体的染色增强，且抗体聚焦的pH范围扩大。C3和F1杂交小鼠中的高应答性还与聚焦抗体染色强度的增加以及pH范围的快速扩大有关。在初次应答早期获取的抗体等电点（pI）值方面还注意到另一个品系差异。B6抗血清所含抗体组分的聚焦区域比C3抗体的更偏碱性。在对无关抗原Taka淀粉酶A（TAA）的应答中，抗体的pI值也出现类似的品系差异，这表明抗体分子的高变区在观察到的品系差异中不起主要作用。F1杂交小鼠的抗血清显示的条带覆盖了B6和C3光谱型的组合pH范围。
