Nakashima S, Kamikawa H
Immunology. 1984 Dec;53(4):837-45.
Changes in the isoelectric focusing (IEF) spectra of specific antibodies were followed during the response of individual mice to bacterial alpha-amylase (B alpha A) in either incomplete or complete Freund's adjuvant (IFA or CFA). The response to a suboptimal dose of B alpha A was maximally enhanced by employing CFA at an optimal dose of Mycobacterium tuberculosis. Regardless of the strain difference in responsiveness to B alpha A between C3H/He (C3) and C57B1/6 (B6) mice, the enhancing effect of CFA was characterized by an accelerated expansion of IEF spectra and by the intensified stain of focused antibody, compared with the response of mice immunized with the same antigen dose in IFA. The manner and the rate of heterogeneity expansion during the enhanced antibody response to a low dose in CFA were quite similar to the strictly restricted expansion of spectra during a response of a high dose of B alpha A in IFA, although each mouse showed an individual banding pattern with a different degree of heterogeneity, depending on the antibody titre. Thus, CFA accelerated the expansion of IEF spectra during the response to B alpha A without affecting the general manner of sequential expansion of anti-B alpha A antibody heterogeneity.
在个体小鼠对细菌α淀粉酶（BαA）在不完全或完全弗氏佐剂（IFA或CFA）中的反应过程中，跟踪特定抗体的等电聚焦（IEF）谱的变化。通过以最佳剂量的结核分枝杆菌使用CFA，对次优剂量BαA的反应得到最大增强。无论C3H/He（C3）和C57B1/6（B6）小鼠对BαA反应性的品系差异如何，与在IFA中用相同抗原剂量免疫的小鼠的反应相比，CFA的增强作用表现为IEF谱的加速扩展和聚焦抗体染色的增强。在CFA中对低剂量的增强抗体反应期间异质性扩展的方式和速率，与在IFA中高剂量BαA反应期间谱的严格受限扩展非常相似，尽管每只小鼠根据抗体滴度显示出具有不同异质性程度的个体条带模式。因此，CFA在对BαA的反应期间加速了IEF谱的扩展，而不影响抗BαA抗体异质性的顺序扩展的一般方式。
