枯草芽孢杆菌168马堡株中一种新的细胞结合型α-淀粉酶的特性，该酶仅与胞外α-淀粉酶存在免疫相关性。
Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.
作者信息
Haddaoui E, Petit-Glatron M F, Chambert R
机构信息
Institut Jacques Monod, Laboratoire Génétique et Membranes, Paris, France.
出版信息
J Bacteriol. 1995 Sep;177(17):5148-50. doi: 10.1128/jb.177.17.5148-5150.1995.
Abstract
Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.
摘要
用针对纯化的胞外α-淀粉酶产生的多克隆抗体对枯草芽孢杆菌细胞提取物进行免疫印迹分析，结果显示有一种82,000道尔顿的蛋白质。即使在编码胞外α-淀粉酶的amyE基因被破坏的细胞中也能发现这种蛋白质。从膜组分中分离出的82,000道尔顿（相对分子质量）的蛋白质在体外表现出α-淀粉酶活性。
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