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1
Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.枯草芽孢杆菌168马堡株中一种新的细胞结合型α-淀粉酶的特性，该酶仅与胞外α-淀粉酶存在免疫相关性。
J Bacteriol. 1995 Sep;177(17):5148-50. doi: 10.1128/jb.177.17.5148-5150.1995.
2
Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis.来自枯草芽孢杆菌的膜结合型和可溶性细胞外α-淀粉酶。
J Biol Chem. 1979 Sep 10;254(17):8540-7.
3
High-level expression, purification and characterization of a recombinant medium-temperature alpha-amylase from Bacillus subtilis.枯草芽孢杆菌中重组中温α-淀粉酶的高效表达、纯化及性质鉴定。
Biotechnol Lett. 2010 Jan;32(1):119-24. doi: 10.1007/s10529-009-0112-4. Epub 2009 Sep 1.
4
Expression and secretion of an acid-stable alpha-amylase gene in Bacillus Subtilis by SacB promoter and signal peptide.利用SacB启动子和信号肽在枯草芽孢杆菌中表达和分泌酸稳定α-淀粉酶基因
Biotechnol Lett. 2005 Nov;27(21):1731-7. doi: 10.1007/s10529-005-2743-4.
5
The purification of a novel amylase from Bacillus subtilis and its inhibition by wheat proteins.从枯草芽孢杆菌中纯化一种新型淀粉酶及其受小麦蛋白的抑制作用
Biochem J. 1983 Feb 1;209(2):561-4. doi: 10.1042/bj2090561.
6
Molecular cloning, nucleotide sequencing, and expression of the Bacillus subtilis (natto) IAM1212 alpha-amylase gene, which encodes an alpha-amylase structurally similar to but enzymatically distinct from that of B. subtilis 2633.枯草芽孢杆菌（纳豆芽孢杆菌）IAM1212 α-淀粉酶基因的分子克隆、核苷酸测序及表达，该基因编码一种结构上与枯草芽孢杆菌2633的α-淀粉酶相似但酶活性不同的α-淀粉酶。
J Bacteriol. 1990 Sep;172(9):4901-8. doi: 10.1128/jb.172.9.4901-4908.1990.
7
[Purification and properties of alpha-amylases in morphological variants of Bacillus subtilis].[枯草芽孢杆菌形态变异体中α-淀粉酶的纯化及性质]
Prikl Biokhim Mikrobiol. 1984 Nov-Dec;20(6):804-9.
8
Modulation of Bacillus subtilis alpha-amylase promoter activity by the presence of a palindromic sequence in front of the gene.基因前回文序列的存在对枯草芽孢杆菌α-淀粉酶启动子活性的调控
Biochem Biophys Res Commun. 1987 Jul 15;146(1):73-9. doi: 10.1016/0006-291x(87)90692-9.
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Cloning in Bacillus subtilis of an extremely thermostable alpha amylase: comparison with other cloned heatstable alpha amylases.一种极端耐热α淀粉酶在枯草芽孢杆菌中的克隆：与其他克隆的耐热α淀粉酶的比较。
Biochem Biophys Res Commun. 1984 Jul 18;122(1):175-83. doi: 10.1016/0006-291x(84)90456-x.
10
Gene cloning and characterization of a thermostable organic-tolerant α-amylase from Bacillus subtilis DR8806.枯草芽孢杆菌DR8806中一种耐热耐有机溶剂α-淀粉酶的基因克隆与特性分析
Int J Biol Macromol. 2015 Jan;72:290-8. doi: 10.1016/j.ijbiomac.2014.08.023. Epub 2014 Aug 26.

本文引用的文献

1
Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.枯草芽孢杆菌细胞外碱性和中性蛋白酶缺陷型双突变体的构建。
J Bacteriol. 1984 Oct;160(1):442-4. doi: 10.1128/jb.160.1.442-444.1984.
2
Nucleotide sequence of the amylase gene from Bacillus subtilis.枯草芽孢杆菌淀粉酶基因的核苷酸序列。
Nucleic Acids Res. 1983 Jan 25;11(2):237-49. doi: 10.1093/nar/11.2.237.
3
Hyperproduction of exocellular levansucrase by Bacillus subtilis: examination of the phenotype of a sacUh strain.
J Gen Microbiol. 1984 Dec;130(12):3143-52. doi: 10.1099/00221287-130-12-3143.
4
Genetic and biochemical studies on cell-bound alpha-amylase in Bacillus subtilis Marburg.枯草芽孢杆菌马伯格菌株中细胞结合型α-淀粉酶的遗传与生化研究。
J Bacteriol. 1974 Aug;119(2):425-30. doi: 10.1128/jb.119.2.425-430.1974.
5
Secretion of Bacillus subtilis levansucrase: a possible two-step mechanism.
Eur J Biochem. 1987 Mar 2;163(2):379-87. doi: 10.1111/j.1432-1033.1987.tb10810.x.
6
Signal peptide of Bacillus subtilis alpha-amylase.枯草芽孢杆菌α淀粉酶的信号肽
J Biochem. 1989 Sep;106(3):376-82. doi: 10.1093/oxfordjournals.jbchem.a122861.
7
A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.一种在枯草芽孢杆菌染色体中以单拷贝状态可选择的新霉素抗性基因盒。
Nucleic Acids Res. 1989 Jun 12;17(11):4410. doi: 10.1093/nar/17.11.4410.
8
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.蔗糖对枯草芽孢杆菌果聚糖蔗糖酶基因表达的调控以及sacU和sacQ基因对稳态mRNA水平的调控。
J Bacteriol. 1986 Oct;168(1):380-8. doi: 10.1128/jb.168.1.380-388.1986.
9
Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis.来自枯草芽孢杆菌的膜结合型和可溶性细胞外α-淀粉酶。
J Biol Chem. 1979 Sep 10;254(17):8540-7.

枯草芽孢杆菌168马堡株中一种新的细胞结合型α-淀粉酶的特性，该酶仅与胞外α-淀粉酶存在免疫相关性。

Characterization of a new cell-bound alpha-amylase in Bacillus subtilis 168 Marburg that is only immunologically related to the exocellular alpha-amylase.

作者信息

Haddaoui E, Petit-Glatron M F, Chambert R

机构信息

Institut Jacques Monod, Laboratoire Génétique et Membranes, Paris, France.

出版信息

J Bacteriol. 1995 Sep;177(17):5148-50. doi: 10.1128/jb.177.17.5148-5150.1995.

DOI:10.1128/jb.177.17.5148-5150.1995
PMID:7665495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177296/
Abstract

Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.

摘要

用针对纯化的胞外α-淀粉酶产生的多克隆抗体对枯草芽孢杆菌细胞提取物进行免疫印迹分析，结果显示有一种82,000道尔顿的蛋白质。即使在编码胞外α-淀粉酶的amyE基因被破坏的细胞中也能发现这种蛋白质。从膜组分中分离出的82,000道尔顿（相对分子质量）的蛋白质在体外表现出α-淀粉酶活性。