Hutten O, Fateh-Moghadam A, Grosse-Wilde H, Mempel W, Netzel B, Ruppelt W
Klin Wochenschr. 1978 Mar 15;56(6):285-90. doi: 10.1007/BF01489174.
Peripheral blood lymphocytes from 32 patients with defined paraproteinaemia (16 IgG, 9 IgA and 7 IgM) and from 15 healthy donors were studied for their in vitro response to various stimuli, including for unspecific mitogens such as Phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin A (ConA) as well as specific antigens such as purified Tuberculin, Candida, Varidase, Tetanus Toxoid, Vaccinia antigen and Vaccinia-control antigen. Mitogens and antigens were lyophilized in Microtiter plates. The lymphocytes of all tested patient-groups responded (measured by H3-Thymidin-uptake) significantly lower towards the unspecific mitogens than those of the control group. If the patients' lymphocytes were stimulated by the specific antigens, their in vitro response was significantly diminished to candida and vaccinia. Macroglobulinaemia showed significantly lower response to ConA if compared to myelomas of IgG- and IgA-type. No correlation was found between mitogen and antigen response and the serum concentration of the paraproteins or immunoglobulins. The results show that monoclonal gammopathy and especially macroglobulinaemia are associated with abnormalities of the cellular immunity which correlates with the clinical observation of increased fungal and viral infections.
对32例确诊为副蛋白血症患者（16例IgG型、9例IgA型和7例IgM型）以及15名健康供者的外周血淋巴细胞进行研究，观察其对各种刺激的体外反应，这些刺激包括非特异性有丝分裂原，如植物血凝素（PHA）、商陆有丝分裂原（PWM）和刀豆球蛋白A（ConA），以及特异性抗原，如纯化结核菌素、念珠菌、溶菌酶、破伤风类毒素、牛痘抗原和牛痘对照抗原。有丝分裂原和抗原冻干于微量滴定板中。所有受试患者组的淋巴细胞（通过³H-胸腺嘧啶核苷摄取量测定）对非特异性有丝分裂原的反应明显低于对照组。如果患者的淋巴细胞受到特异性抗原刺激，其对念珠菌和牛痘的体外反应明显减弱。与IgG型和IgA型骨髓瘤相比，巨球蛋白血症对ConA的反应明显较低。未发现有丝分裂原和抗原反应与副蛋白或免疫球蛋白的血清浓度之间存在相关性。结果表明，单克隆丙种球蛋白病尤其是巨球蛋白血症与细胞免疫异常有关，这与真菌和病毒感染增加的临床观察结果相关。
