Zimmer M, Scheit K H
Nucleic Acids Res. 1984 Mar 12;12(5):2243-58. doi: 10.1093/nar/12.5.2243.
The effects of the following pyrimidine nucleoside 5'-triphosphates: f5 UTP, br5 UTP, rTTP, s2 UTP, s4 UTP and s2 CTP on cell-free expression of the beta-galactosidase gene in lambda h80dlac DNA as well as the galactokinase gene in plasmid 01-14 were investigated. Only rTTP could substitute UTP in cell-free gene expression without restriction. Combinations of the other analogs with their respective natural congeners led to inhibition of gene expression. All analogs were found to inhibit transcription. Whereas br5 UTP and s4 UTP did not affect translation, mRNA containing s2 UMP or s2 CMP residues respectively was found to function poorly in translation. Only in the case of f5 UTP could ambiguitive behaviour be demonstrated. Whether mispairing of f5 UMP residues, responsible for this ambiguity takes place in transcription or in translation, could not be decided.
研究了以下嘧啶核苷5'-三磷酸：f5 UTP、br5 UTP、rTTP、s2 UTP、s4 UTP和s2 CTP对λh80dlac DNA中β-半乳糖苷酶基因以及质粒01-14中半乳糖激酶基因无细胞表达的影响。只有rTTP可以不受限制地替代UTP用于无细胞基因表达。其他类似物与各自天然同类物的组合导致基因表达受到抑制。发现所有类似物均抑制转录。br5 UTP和s4 UTP不影响翻译，而分别含有s2 UMP或s2 CMP残基的mRNA在翻译中功能不佳。只有在f5 UTP的情况下才能表现出模糊的行为。导致这种模糊性的f5 UMP残基错配是发生在转录还是翻译过程中，尚无法确定。
