有机磷酸盐可提高禽血红蛋白D和鸡胚胎血红蛋白的溶解度。
Organic phosphates increase the solubility of avian haemoglobin D and embryonic chicken haemoglobin.
作者信息
Baumann R, Goldbach E, Haller E A, Wright P G
出版信息
Biochem J. 1984 Feb 1;217(3):767-71. doi: 10.1042/bj2170767.
The isolated minor haemoglobin fractions (haemoglobin D) of ostrich, chicken and duck haemoglobin, which constitute about 30% of total intracellular haemoglobin, form crystalline aggregates upon deoxygenation at physiological temperature, ionic strength and pH and at haemoglobin concentrations even well below those present in the red cell. The aggregation is reversed by oxygenation, and can be inhibited by addition of organic phosphates or the corresponding major haemoglobin fraction in a stoichiometric ratio of 1:1. Embryonic haemoglobin from chicken has similar characteristics with respect to its solubility. The results indicate close functional homology of alpha D and embryonic pi-chains as well as a novel role for organic phosphates in the regulation of haemoglobin function.
鸵鸟、鸡和鸭血红蛋白中分离出的次要血红蛋白组分（血红蛋白D）约占细胞内总血红蛋白的30%，在生理温度、离子强度和pH值下，以及血红蛋白浓度远低于红细胞中的浓度时，脱氧后会形成结晶聚集体。氧合可使聚集逆转，添加有机磷酸盐或以1:1的化学计量比添加相应的主要血红蛋白组分可抑制聚集。鸡胚胎血红蛋白在溶解性方面具有类似特征。结果表明αD链和胚胎π链在功能上具有密切的同源性，以及有机磷酸盐在血红蛋白功能调节中的新作用。
Zotero 插件