Guenounou M, Smets P, Agneray J
C R Acad Sci III. 1984;298(6):135-8.
RU 41740, a glycoprotein extract from K. pneumoniae K2O1 strain, is an immune modulating compound which has been shown to reduce infectious episodes in deficient patients. Data from experimental designs suggest that RU 41740 could affect several target cells, such as T cells, B cells or macrophages. In the present report we show that RU 41740 is a selective B cell activator. It induces blast transformation in normal and nude mouse spleen cells and does not activate mouse T lymphocytes to proliferate. Activation of mouse spleen cells by RU 41740 is not affected by removal of adherent cells. RU 41740 also activates immunoglobulin secretion by murine B lymphocytes. Incubating spleen cells from C3H/HeJ mice with RU 41740 results in cell proliferation and activation of antibody forming cells. This suggests that B cell activation is not due to LPS contamination.
RU 41740是一种从肺炎克雷伯菌K2O1菌株中提取的糖蛋白提取物，是一种免疫调节化合物，已被证明可减少免疫缺陷患者的感染发作次数。实验设计数据表明，RU 41740可能影响多种靶细胞，如T细胞、B细胞或巨噬细胞。在本报告中，我们表明RU 41740是一种选择性B细胞激活剂。它可诱导正常和裸鼠脾细胞发生原始细胞转化，且不会激活小鼠T淋巴细胞增殖。去除贴壁细胞不会影响RU 41740对小鼠脾细胞的激活作用。RU 41740还可激活小鼠B淋巴细胞分泌免疫球蛋白。用RU 41740孵育C3H/HeJ小鼠的脾细胞会导致细胞增殖和抗体形成细胞的激活。这表明B细胞激活并非由脂多糖污染所致。
