Papa K E
Can J Microbiol. 1984 Jan;30(1):68-73. doi: 10.1139/m84-012.
Eight aflatoxin (afl) mutants of Aspergillus flavus were induced with N-methyl-N'-nitro-N-nitrosoguanidine. Heterozygous diploids formed between afl mutants and tester strains revealed that each afl mutant was recessive. Haploids selected from these heterozygous diploids indicated the linkage of all eight afl mutants to markers on group VII. These include previously mapped arg-7 (arginine), leu (leucine), dominant afl-1, and nor which accumulates norsolorinic acid that is visible as an orange-red pigment. Diploid complementation tests indicated that all but two afl mutants were nonallelic. Diploids homozygous for nor, resulting from crossing-over, were isolated and used to map new afl genes.
用N-甲基-N'-硝基-N-亚硝基胍诱导了8个黄曲霉的黄曲霉毒素（afl）突变体。afl突变体与测试菌株之间形成的杂合二倍体表明，每个afl突变体都是隐性的。从这些杂合二倍体中选出的单倍体表明，所有8个afl突变体都与第VII组的标记连锁。这些标记包括先前定位的arg-7（精氨酸）、leu（亮氨酸）、显性afl-1，以及积累诺索洛林酸的nor，诺索洛林酸以橙红色色素形式可见。二倍体互补试验表明，除两个afl突变体外，其余均为非等位基因。通过交叉产生的nor纯合二倍体被分离出来，并用于定位新的afl基因。
