腐生酵母与黄曲霉nor突变体的相互作用。
Interactions of saprophytic yeasts with a nor mutant of Aspergillus flavus.
作者信息
Hua S S, Baker J L, Flores-Espiritu M
机构信息
Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710, USA.
出版信息
Appl Environ Microbiol. 1999 Jun;65(6):2738-40. doi: 10.1128/AEM.65.6.2738-2740.1999.
Abstract
The nor mutant of Aspergillus flavus has a defective norsolorinic acid reductase, and thus the aflatoxin biosynthetic pathway is blocked, resulting in the accumulation of norsolorinic acid, a bright red-orange pigment. We developed a visual agar plate assay to monitor yeast strains for their ability to inhibit aflatoxin production by visually scoring the accumulation of this pigment of the nor mutant. We identified yeast strains that reduced the red-orange pigment accumulation in the nor mutant. These yeasts also reduced aflatoxin accumulation by a toxigenic strain of A. flavus. These yeasts may be useful for reducing aflatoxin contamination of food commodities.
摘要
黄曲霉的nor突变体具有缺陷的诺尔洛菌素酸还原酶，因此黄曲霉毒素生物合成途径被阻断，导致诺尔洛菌素酸（一种亮红橙色色素）积累。我们开发了一种视觉琼脂平板测定法，通过对nor突变体这种色素的积累进行视觉评分，来监测酵母菌株抑制黄曲霉毒素产生的能力。我们鉴定出了能减少nor突变体中红橙色色素积累的酵母菌株。这些酵母还减少了黄曲霉产毒菌株的黄曲霉毒素积累。这些酵母可能有助于减少食品商品中的黄曲霉毒素污染。
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