Bennett J W
J Gen Microbiol. 1979 Jul;113(1):127-36. doi: 10.1099/00221287-113-1-127.
Three spore colour, two mycelial colour and 12 auxotrophic mutants were isolated from an aflatoxigenic strain of Aspergillus parasiticus. These mutants and heterozygous diploids formed by pairwise combinations of auxotrophs were assayed for aflatoxin production; norsolorinic acid and versicolorin A production were also assayed in the diploids. In general, introduction of an auxotrophic marker lowered aflatoxin production in haploids. The green-spored, prototrophic diploids resembled haploid wild-type strains in that they produced high levels of aflatoxin, low levels of versicolorin A, and no detectable norsolorinic acid. Parasexual analysis of segregants from four heterozygous diploids was hampered by the uniform conidiospore diameter of haploids and diploids and by the non-random recovery of genotypes among somatic segregants with and without treatment with p-fluorophenylalanine. Nevertheless, this technique is useful for recombinational analysis of mutants blocked in aflatoxin synthesis. The fortuitous association of mycelial pigmentation with certain blocked aflatoxin mutants should prove useful in future analyses of the genetics and biosynthesis of these economically important secondary metabolites.
从寄生曲霉的一株产黄曲霉毒素菌株中分离出了三种孢子颜色、两种菌丝体颜色和12个营养缺陷型突变体。对这些突变体以及由营养缺陷型两两组合形成的杂合二倍体进行了黄曲霉毒素产生情况的检测；同时也检测了二倍体中柄曲霉素和杂色曲霉素A的产生情况。一般来说，引入营养缺陷型标记会降低单倍体中黄曲霉毒素的产量。绿色孢子的原养型二倍体与单倍体野生型菌株相似，它们产生高水平的黄曲霉毒素、低水平的杂色曲霉素A，且检测不到柄曲霉素。来自四个杂合二倍体的分离子的准性分析受到单倍体和二倍体分生孢子直径一致以及经对氟苯丙氨酸处理和未处理的体细胞分离子中基因型非随机恢复的阻碍。然而，该技术对于黄曲霉毒素合成受阻的突变体的重组分析是有用的。菌丝体色素沉着与某些受阻的黄曲霉毒素突变体的偶然关联在未来对这些具有经济重要性的次生代谢产物的遗传学和生物合成分析中应会证明是有用的。
