English L S, Whitehurst M
Cell Immunol. 1984 May;85(2):364-72. doi: 10.1016/0008-8749(84)90250-8.
Lymph and supernatants derived from efferent lymphocytes leaving the popliteal lymph nodes of sheep responding to human red cells or dinitrophenylated bovine serum albumin were examined for the presence of T-cell growth factor (TCGF). Efferent cells from normal sheep, but not from antigen-stimulated sheep, were found to release low levels of TCGF when incubated in medium for 12 hr in the absence of any exogenous stimulus. High levels of TCGF were found in normal lymph and also in immune lymph collected from sheep during the first 6 hr of immune responses. There were no detectable levels of TCGF in lymph collected later in the response. The lymphokine appeared to be a single molecular species of 10,000-20,000 molecular weight as assessed by exclusion chromatography. Efferent cells expressing receptors for TCGF were found in efferent lymph during the first 12 hr of the response. The results demonstrate for the first time that TCGF is produced in vivo and that asynchrony exists between TCGF production and expression of receptors for TCGF on efferent cells released by the stimulated node. Based on the known kinetics of previously reported synergistic factors, mitogenic factors, and T-cell-replacing factors in sheep efferent lymph and their physical characteristics it was concluded that the TCGF detected in lymph is distinct from these factors.
对源自绵羊腘淋巴结、对人红细胞或二硝基苯基化牛血清白蛋白产生反应的传出淋巴细胞的淋巴液和上清液进行检测，以确定是否存在T细胞生长因子（TCGF）。结果发现，正常绵羊的传出细胞在无任何外源性刺激的培养基中孵育12小时后会释放低水平的TCGF，而抗原刺激后的绵羊传出细胞则不会。在正常淋巴液以及免疫反应最初6小时内从绵羊采集的免疫淋巴液中发现了高水平的TCGF。在反应后期采集的淋巴液中未检测到TCGF。通过排阻色谱法评估，这种淋巴因子似乎是一种分子量为10,000 - 20,000的单一分子。在反应的最初12小时内，在传出淋巴液中发现了表达TCGF受体的传出细胞。这些结果首次证明TCGF在体内产生，并且在刺激淋巴结释放的传出细胞上，TCGF的产生与TCGF受体的表达之间存在不同步现象。基于先前报道的绵羊传出淋巴液中协同因子、促有丝分裂因子和T细胞替代因子的已知动力学及其物理特性，得出结论：在淋巴液中检测到的TCGF与这些因子不同。
