Heino J, Rajamäki A, Irjala K
Scand J Clin Lab Invest. 1984 Apr;44(2):173-6. doi: 10.3109/00365518409161401.
The different polymeric forms of free kappa and lambda light chains from urines of two myeloma patients were separated by gel filtration. When samples containing kappa chains in equal protein concentration were quantified by turbidimetry with anti-free kappa chain antibodies, two out of three samples reacted much less sensitively than the third sample. When analysed with SDS-polyacrylamide gel electrophoresis in conditions dissociating non-covalent but not covalent linkages, these two samples contained monomeric light chains, whereas the third sample contained dimers.
通过凝胶过滤分离了两名骨髓瘤患者尿液中游离κ和λ轻链的不同聚合形式。当用抗游离κ链抗体通过比浊法对蛋白质浓度相等的含κ链样品进行定量时，三个样品中有两个的反应灵敏度远低于第三个样品。在解离非共价但不解离共价键的条件下用SDS-聚丙烯酰胺凝胶电泳分析时，这两个样品含有单体轻链，而第三个样品含有二聚体。
