Sölling K
Scand J Clin Lab Invest. 1976 Sep;36(5):447-52. doi: 10.3109/00365517609054462.
The polymeric forms of free light chains from the immunoglobulins have been estimated in serum from 10 healthy individuals and from 10 anephric patients. Light chains were also estimated in serum and urine from 29 patients with various degrees of renal insufficiency. The measurements were carried out by a radioimmunoassay. The mean concentrations of light chains in normal serum were found to be 4.9 mg/l for dimeric forms of kappa chains, 5.6 mg/l for monomeric forms of kappa chains, 5.1 mg/l for dimeric forms of lambda chains, and 2.7 mg/l for monomeric forms of lambda chains. The concentration of light chains in anephric man was increased to 5 times the normal level. The concentration of the different forms was 26.7 mg/l for dimeric forms of kappa chains, 29.6 mg/l for monomeric forms of kappa chains, 32.6 mg/l for dimeric forms of lambda chains, and 5.8 mg/l for monomeric forms of lambda chains. A minor amount of tetrameric forms of kappa chains was found. Gel filtration showed that a majority of the kappa chains in normal and anephric serum existed as monomers and non-covalently linked dimers, whereas the lambda chains mainly existed as stable, covalently linked dimers. In renal disease the serum concentration of light chains was found closely correlated to creatinine clearance. The 24-h urinary excretion of light chains was generally increased when the GFR was diminished regardless of the type of renal disease.
已对10名健康个体和10名无肾患者血清中的免疫球蛋白游离轻链聚合物形式进行了评估。还对29名不同程度肾功能不全患者的血清和尿液中的轻链进行了评估。测量采用放射免疫分析法进行。发现正常血清中轻链的平均浓度为：κ链二聚体形式4.9mg/L，κ链单体形式5.6mg/L，λ链二聚体形式5.1mg/L，λ链单体形式2.7mg/L。无肾男性轻链浓度增加至正常水平的5倍。不同形式的浓度为：κ链二聚体形式26.7mg/L，κ链单体形式29.6mg/L，λ链二聚体形式32.6mg/L，λ链单体形式5.8mg/L。发现少量κ链四聚体形式。凝胶过滤显示，正常血清和无肾血清中的大多数κ链以单体和非共价连接的二聚体形式存在，而λ链主要以稳定的共价连接二聚体形式存在。在肾脏疾病中，发现血清轻链浓度与肌酐清除率密切相关。无论肾脏疾病类型如何，当肾小球滤过率降低时，轻链的24小时尿排泄量通常会增加。
